• 1. Fitzpatrick GM, Cliff R, Tandon N. Thrombosomes: a platelet-derived hemostatic agent for control of non-compressible hemorrhage. Transfusion 2013;53:100S106S.

    • Search Google Scholar
    • Export Citation
  • 2. Hawksworth JS, Elster EA, Fryer D, et al. Evaluation of lyophilized platelets as an infusible hemostatic agent in experimental non-compressible hemorrhage in swine. J Thromb Haemost 2009;7:16631671.

    • Search Google Scholar
    • Export Citation
  • 3. Holcomb JB, Wade CE, Michalek JE, et al. Increased plasma and platelet to red blood cell ratios improves outcome in 466 massively transfused civilian trauma patients. Ann Surg 2008;248:447458.

    • Search Google Scholar
    • Export Citation
  • 4. Hux BD, Martin LG. Platelet transfusions: treatment options for hemorrhage secondary to thrombocytopenia. J Vet Emerg Crit Care (San Antonio) 2012;22:7380.

    • Search Google Scholar
    • Export Citation
  • 5. Latimer E, Zong JC, Haeggans SY, et al. Detection and evaluation of novel herpesvirus in routine and pathological samples from Asian and African elephants: identification of two new probosciviruses (EEHV5 and EEHV6) and two new gammaherpesviruses (EGHV3B and EGHV5). Vet Microbiol 2011;147:2841.

    • Search Google Scholar
    • Export Citation
  • 6. Zink KA, Sambasivan CN, Holcomb JB, et al. A high ratio of plasma and platelets to packed red blood cells in the first 6 hours of massive transfusion improves outcomes in a large multicenter study. Am J Surg 2009;197:565570.

    • Search Google Scholar
    • Export Citation
  • 7. Bode AP, Fischer TH. Lyophilized platelets: 50 years in the making. Artif Cells Blood Substit Immobil Biotechnol 2007;35:125133.

  • 8. Cap AP, Perkins JG. Lyophilized platelets: challenges and opportunities. J Trauma 2011;70:S59S60.

  • 9. Pietramaggiori G, Kaipainen A, Ho D, et al. Trehalose lyophilized platelets for wound healing. Wound Repair Regen 2007;15:213220.

  • 10. Davidow EB, Brainard B, Martin LG, et al. Use of fresh platelet concentrate or lyophilized platelets in thrombocytopenic dogs with clinical signs of hemorrhage: a preliminary trial in 37 dogs. J Vet Emerg Crit Care (San Antonio) 2012;22:116125.

    • Search Google Scholar
    • Export Citation
  • 11. McNicol A, Israels SJ. Beyond hemostasis: the role of platelets in inflammation, malignancy and infection. Cardiovasc Hematol Disord Drug Targets 2008;8:99117.

    • Search Google Scholar
    • Export Citation
  • 12. Ariede JR, Pardini MIMC, Silva GF, et al. Platelets can be a biological compartment for the hepatitis C virus. Braz J Microbiol 2015;46:627629.

    • Search Google Scholar
    • Export Citation
  • 13. Getz TM, Montgomery RK, Bynum JA, et al. Storage of platelets at 4C in platelet additive solutions prevents aggregate formation and preserves platelet functional responses. Transfusion 2016;56:13201328.

    • Search Google Scholar
    • Export Citation
  • 14. Handigund M, Bae TW, Lee J, et al. Evaluation of in vitro storage characteristics of cold stored platelet concentrates with N-acetylcysteine (NAC). Transfus Apher Sci 2016;54:127138.

    • Search Google Scholar
    • Export Citation
  • 15. Currie LM, Harper JR, Allan H, et al. Inhibition of cytokine accumulation and bacterial growth during storage of platelet concentrates at 4 degrees C with retention of in vitro functional activity. Transfusion 1997;37:1824.

    • Search Google Scholar
    • Export Citation
  • 16. Wolkers WF, Walker NJ, Tablin F, et al. Human platelets loaded with trehalose survive freeze-drying. Cryobiology 2001;42:7987.

  • 17. Sum R, Hager S, Pietramaggiori G, et al. Wound-healing properties of trehalose-stabilized freeze-dried outdated platelets. Transfusion 2007;47:672679.

    • Search Google Scholar
    • Export Citation
  • 18. Fischer TH, Bode AP, Parker BR, et al. Primary and secondary hemostatic functionalities of rehydrated, lyophilized platelets. Transfusion 2006;46:19431950.

    • Search Google Scholar
    • Export Citation
  • 19. Slichter S, Fitzpatrick GM. Evaluation of the safety and immunogenicity of autologous thrombosomes in healthy human subjects; a microdose escalation study (cohorts 1–4) and repeat microdose immunogenicity study (cohort 5). Available at: https://clinicaltrials.gov/ct2/show/NCT02223117. Accessed Sep 28, 2016.

    • Search Google Scholar
    • Export Citation
  • 20. Bode AP, Lust RM, Read MS, et al. Correction of the bleeding time with lyophilized platelet infusions in dogs on cardiopulmonary bypass. Clin Appl Thromb Hemost 2008;14:3854.

    • Search Google Scholar
    • Export Citation
  • 21. Cap AP, Getz TM, Spinella PC, et al. Platelet transfusion. In: Gonzalez E, Moore HB, Moore EE, eds. Trauma induced coagulopathy. Basel, Switzerland: Springer; 2016;347376.

    • Search Google Scholar
    • Export Citation
  • 22. Fischer TH, Wolberg AS, Bode AP, et al. The interaction of factor VIIa with rehydrated, lyophilized platelets. Platelets 2008;19:182191.

    • Search Google Scholar
    • Export Citation
  • 23. Macko AR, Crossland RF, Cap AP, et al. Control of severe intra-abdominal hemorrhage with an infusible platelet-derived hemostatic agent in nonhuman primate (rhesus macaque) model. J Trauma Acute Care Surg 2016;80:617624.

    • Search Google Scholar
    • Export Citation
  • 24. Zapata JC, Cox D, Salvato MS. The role of platelets in the pathogenesis of viral hemorrhagic fevers. PLoS Negl Trop Dis 2014;8:e2858.

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Do lyophilized platelets hold promise for treatment of hemorrhagic diseases in wild animals?

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  • 1 Smithsonian Global Health Program, Smithsonian Conservation Biology Institute, National Zoological Park, 3001 Connecticut Ave NW, Washington, DC 20013.
  • | 2 Smithsonian Global Health Program, Smithsonian Conservation Biology Institute, National Zoological Park, 3001 Connecticut Ave NW, Washington, DC 20013.
  • | 3 Cellphire Inc, 9430 Key W Ave, Rockville, MD 20850.
  • | 4 Smithsonian Global Health Program, Smithsonian Conservation Biology Institute, National Zoological Park, 3001 Connecticut Ave NW, Washington, DC 20013.
  • | 5 San Diego Wild Animal Park, 15500 San Pasqual Valley Rd, Escondido, CA 92027.
  • | 6 Ringling Bros. Center for Elephant Conservation, 12850 Old Grade Road, Polk City, FL 33868.
  • | 7 Fort Worth Zoo, 1989 Colonial Pkwy, Fort Worth, TX 76110.
  • | 8 Smithsonian Global Health Program, Smithsonian Conservation Biology Institute, National Zoological Park, 3001 Connecticut Ave NW, Washington, DC 20013.

Contributor Notes

Address correspondence to Dr. Kishbaugh (kishbaughj@si.edu).