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Evaluation of serum biochemical marker concentrations and survival time in dogs with protein-losing enteropathy

Mirjam Equilino DVM1, Vincent Théodoloz DVM2, Daniela Gorgas DVM, Dr med vet3, Marcus G. Doherr DVM, Dr med vet, PhD4, Romy M. Heilmann DVM, Dr med vet5, Jan S. Suchodolski DVM, Dr med vet, PhD6, Jörg M. Steiner Dr med vet, PhD7, and Iwan A. Burgener DVM Dr med vet, PhD, Dr Habil8
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  • 1 Department of Clinical Veterinary Medicine, Vetsuisse Faculty, University of Bern, Bern, 3001 Switzerland.
  • | 2 Department of Clinical Veterinary Medicine, Vetsuisse Faculty, University of Bern, Bern, 3001 Switzerland.
  • | 3 Department of Clinical Veterinary Medicine, Vetsuisse Faculty, University of Bern, Bern, 3001 Switzerland.
  • | 4 Department of Clinical Research and Veterinary Public Health, Vetsuisse Faculty, University of Bern, Bern, 3001 Switzerland.
  • | 5 Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843.
  • | 6 Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843.
  • | 7 Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843.
  • | 8 Division of Small Animal Internal Medicine, Faculty of Veterinary Medicine, University of Leipzig, Leipzig, 04103 Germany.

Abstract

Objective—To evaluate serum concentrations of biochemical markers and survival time in dogs with protein-losing enteropathy (PLE).

Design—Prospective study.

Animals—29 dogs with PLE and 18 dogs with food-responsive diarrhea (FRD).

Procedures—Data regarding serum concentrations of various biochemical markers at the initial evaluation were available for 18 of the 29 dogs with PLE and compared with findings for dogs with FRD. Correlations between biochemical marker concentrations and survival time (interval between time of initial evaluation and death or euthanasia) for dogs with PLE were evaluated.

Results—Serum C-reactive protein concentration was high in 13 of 18 dogs with PLE and in 2 of 18 dogs with FRD. Serum concentration of canine pancreatic lipase immunoreactivity was high in 3 dogs with PLE but within the reference interval in all dogs with FRD. Serum α1-proteinase inhibitor concentration was less than the lower reference limit in 9 dogs with PLE and 1 dog with FRD. Compared with findings in dogs with FRD, values of those 3 variables in dogs with PLE were significantly different. Serum calprotectin (measured by radioimmunoassay and ELISA) and S100A12 concentrations were high but did not differ significantly between groups. Seventeen of the 29 dogs with PLE were euthanized owing to this disease; median survival time was 67 days (range, 2 to 2,551 days).

Conclusions and Clinical Relevance—Serum C-reactive protein, canine pancreatic lipase immunoreactivity, and α1-proteinase inhibitor concentrations differed significantly between dogs with PLE and FRD. Most initial biomarker concentrations were not predictive of survival time in dogs with PLE.

Abstract

Objective—To evaluate serum concentrations of biochemical markers and survival time in dogs with protein-losing enteropathy (PLE).

Design—Prospective study.

Animals—29 dogs with PLE and 18 dogs with food-responsive diarrhea (FRD).

Procedures—Data regarding serum concentrations of various biochemical markers at the initial evaluation were available for 18 of the 29 dogs with PLE and compared with findings for dogs with FRD. Correlations between biochemical marker concentrations and survival time (interval between time of initial evaluation and death or euthanasia) for dogs with PLE were evaluated.

Results—Serum C-reactive protein concentration was high in 13 of 18 dogs with PLE and in 2 of 18 dogs with FRD. Serum concentration of canine pancreatic lipase immunoreactivity was high in 3 dogs with PLE but within the reference interval in all dogs with FRD. Serum α1-proteinase inhibitor concentration was less than the lower reference limit in 9 dogs with PLE and 1 dog with FRD. Compared with findings in dogs with FRD, values of those 3 variables in dogs with PLE were significantly different. Serum calprotectin (measured by radioimmunoassay and ELISA) and S100A12 concentrations were high but did not differ significantly between groups. Seventeen of the 29 dogs with PLE were euthanized owing to this disease; median survival time was 67 days (range, 2 to 2,551 days).

Conclusions and Clinical Relevance—Serum C-reactive protein, canine pancreatic lipase immunoreactivity, and α1-proteinase inhibitor concentrations differed significantly between dogs with PLE and FRD. Most initial biomarker concentrations were not predictive of survival time in dogs with PLE.

Contributor Notes

Dr. Equilino's present address is Tierklinik Thun-Süd AG, Burgerstrasse 11, 3600 Thun, Switzerland.

Presented as an oral abstract at the 29th Forum of the American College of Veterinary Internal Medicine, Denver, June 2011.

This study was not supported by a grant, and no conflicts of interest were reported.

Address correspondence to Dr. Burgener (I.Burgener@uu.nl).