• 1. Mayer J, Wagner R, Taeymans O. Advanced diagnostic approaches and current management of thyroid pathologies in guinea pigs. Vet Clin North Am Exot Anim Pract 2010; 13:509523.

    • Search Google Scholar
    • Export Citation
  • 2. Garner M. A pictorial of selected diseases in guinea pigs. Exotic DVM 2006; 8:4850.

  • 3. Ewringmann A, Glöckner B. Leitsymptome bei Meerschweinchen, Chinchilla und Degu. Diagnostischer Leitfaden und Therapie. Stuttgart, Germany: Enke Publishing, 2005; 7577.

    • Search Google Scholar
    • Export Citation
  • 4. Castro MI, Alex S, Young RA, et al. Total and free plasma thyroid hormone concentrations in fetal and adult pregnant and non-pregnant guinea pigs. Endocrinology 1986; 118:533537.

    • Search Google Scholar
    • Export Citation
  • 5. Müller K, Müller E, Klein R, et al. Plasma thyroxine concentrations in clinically healthy pet guinea pigs (Cavia porcellus). Vet Clin Pathol 2009; 38:507510.

    • Search Google Scholar
    • Export Citation
  • 6. Stegeman JR, Graham PA, Hauptman JG. Use of recombinant human thyroid-stimulating hormone for thyrotropin-stimulation testing of euthyroid cats. Am J Vet Res 2003; 64:149152.

    • Search Google Scholar
    • Export Citation
  • 7. Sauvé F, Paradis M. Use of recombinant human thyroid-stimulating hormone for thyrotropin stimulation test in euthyroid dogs. Can Vet J 2000; 41:215219.

    • Search Google Scholar
    • Export Citation
  • 8. Cunningham JG. Textbook of veterinary physiology. 4th ed. Philadelphia: WB Saunders Co, 2007; 428436.

  • 9. Feldman EC, Nelson RW. Canine and feline endocrinology and reproduction. 3rd ed. Philadelphia: WB Saunders Co, 2004; 85152.

  • 10. Ribela MT, Bianco AC, Bartolini P. The use of recombinant human thyrotropin produced by Chinese hamster ovary cells for the preparation of immuneassay reagents. J Clin Endocrinol Metab 1996; 81:249256.

    • Search Google Scholar
    • Export Citation
  • 11. Meier CA, Braverman LE, Ebner SA, et al. Diagnostic use of recombinant human thyrotropin in patients with thyroid carcinoma (phase I/II study). J Clin Endocrinol Metab 1994; 78:188196.

    • Search Google Scholar
    • Export Citation
  • 12. Colzani RM, Alex S, Fang SL, et al. The effect of recombinant human thyrotropin (rhTSH) on thyroid function in mice and rats. Thyroid 1998; 8:797801.

    • Search Google Scholar
    • Export Citation
  • 13. Chastain CB, Ganjam VK. Clinical endocrinology of companion animals. Philadelphia: Lea & Febiger, 1986; 113153.

  • 14. Genzyme Corp. Thyrogen, thyrotropin alfa for injection. www.thyrogen.com/patient/about/thy_pt_safety_information.asp. Accessed Apr 13, 2011.

    • Search Google Scholar
    • Export Citation
  • 15. Shiel RE, Mooney CT. Testing for hyperthyroidism in cats. Vet Clin North Am Small Anim Pract 2007; 37:671691.

  • 16. Peterson ME, Kintzer PP, Cavanagh PG, et al. Feline hyperthyroidism: pretreatment clinical and laboratory evaluation of 131 cases. J Am Vet Med Assoc 1983; 183:103110.

    • Search Google Scholar
    • Export Citation
  • 17. Stegeman JR, Graham PA, Hauptman JG. Use of recombinant human thyroid-stimulating hormone for thyrotropin-stimulation testing of euthyroid cats. Am J Vet Res 2003; 64:149152.

    • Search Google Scholar
    • Export Citation
  • 18. Russell NJ, Foster S, Clark P, et al. Comparison of radioimmunoassay and chemiluminescent assay methods to estimate canine blood cortisol concentrations. Aust Vet J 2007; 85:487494.

    • Search Google Scholar
    • Export Citation
  • 19. Chapwanya A, Clegg T, Stanley P, et al. Comparison of Immulite and RIA assay methods for measuring peripheral blood progesterone levels in Greyhound bitches. Theriogenology 2008; 70:795799.

    • Search Google Scholar
    • Export Citation

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Use of recombinant human thyroid-stimulating hormone for evaluation of thyroid function in guinea pigs (Cavia porcellus)

Jörg Mayer Dr Med Vet, MSC, DABVP1, Robert Wagner VMD, DABVP2, Mark A. Mitchell DVM, PhD3, and Kellie Fecteau PhD4
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  • 1 Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, University of Georgia, Athens, GA 30602.
  • | 2 Division of Laboratory Animal Services, University of Pittsburgh, Pittsburgh, PA 15261.
  • | 3 Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois, Urbana, IL 61802.
  • | 4 Clinical Endocrinology Service, Department of Comparative Medicine, College of Veterinary Medicine, University of Tennessee, Knoxville, TN 37996.

Abstract

Objective—To evaluate the effects of administration of recombinant human (rh) thyroid-stimulating hormone (TSH) for evaluation of thyroid function in euthyroid guinea pigs (Cavia porcellus).

Design—Prospective, experimental study.

Animals—10 healthy, sexually intact, pet guinea pigs (approx 1 year of age).

Procedures—Guinea pigs were given rhTSH (100 μg, IM); plasma thyroxine concentrations were determined prior to and 3 and 4 hours after rhTSH injection. The animals were housed in 2 groups on the basis of sex and fed different commercial maintenance diets according to their husbandry.

Results—There was no significant difference in thyroxine concentrations between males and females before or after rhTSH injection. There was also no difference between thyroxine concentrations at 3 versus 4 hours after rhTSH injection. There was a significant difference between thyroxine concentrations before (median, 9.05 nmol/L [0.70 μg/dL]; 10% to 90% range, 7.39 to 16.99 nmol/L [0.57 to 1.32 μg/dL]) and after (mean ± SD, 23.95 ± 4.2 nmol/L) rhTSH injection. Euthyroid guinea pigs had plasma thyroxine concentrations of at least 7.30 nmol/L (0.57 μg/dL) and an increase of at least 2.6 times prestimulation thyroxine concentrations at 3 or 4 hours after stimulation.

Conclusions and Clinical Relevance—The results suggested that rhTSH administered IM can be used for the TSH stimulation testing in guinea pigs. We suggest thyroxine concentration in a euthyroid guinea pig should at least double 3 to 4 hours after rhTSH injection.

Abstract

Objective—To evaluate the effects of administration of recombinant human (rh) thyroid-stimulating hormone (TSH) for evaluation of thyroid function in euthyroid guinea pigs (Cavia porcellus).

Design—Prospective, experimental study.

Animals—10 healthy, sexually intact, pet guinea pigs (approx 1 year of age).

Procedures—Guinea pigs were given rhTSH (100 μg, IM); plasma thyroxine concentrations were determined prior to and 3 and 4 hours after rhTSH injection. The animals were housed in 2 groups on the basis of sex and fed different commercial maintenance diets according to their husbandry.

Results—There was no significant difference in thyroxine concentrations between males and females before or after rhTSH injection. There was also no difference between thyroxine concentrations at 3 versus 4 hours after rhTSH injection. There was a significant difference between thyroxine concentrations before (median, 9.05 nmol/L [0.70 μg/dL]; 10% to 90% range, 7.39 to 16.99 nmol/L [0.57 to 1.32 μg/dL]) and after (mean ± SD, 23.95 ± 4.2 nmol/L) rhTSH injection. Euthyroid guinea pigs had plasma thyroxine concentrations of at least 7.30 nmol/L (0.57 μg/dL) and an increase of at least 2.6 times prestimulation thyroxine concentrations at 3 or 4 hours after stimulation.

Conclusions and Clinical Relevance—The results suggested that rhTSH administered IM can be used for the TSH stimulation testing in guinea pigs. We suggest thyroxine concentration in a euthyroid guinea pig should at least double 3 to 4 hours after rhTSH injection.

Contributor Notes

Address correspondence to Dr. Mayer (mayerj@uga.edu).