Collection and analysis of peritoneal fluid from healthy llamas and alpacas

Christopher K. Cebra Departments of Clinical Sciences and Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331-4802.

Search for other papers by Christopher K. Cebra in
Current site
Google Scholar
PubMed
Close
 VMD, MS, DACVIM
,
Susan J. Tornquist Departments of Clinical Sciences and Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331-4802.

Search for other papers by Susan J. Tornquist in
Current site
Google Scholar
PubMed
Close
 DVM, PhD, DACVP
, and
Shannon K. Reed Departments of Clinical Sciences and Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331-4802.

Search for other papers by Shannon K. Reed in
Current site
Google Scholar
PubMed
Close
 DVM

Abstract

Objective—To describe a technique for abdominocentesis in camelids and report peritoneal fluid biochemical and cytologic findings from healthy llamas and alpacas.

Design—Prospective study.

Animals—17 adult llamas and 5 adult alpacas.

Procedures—Right paracostal abdominocentesis was performed. Peritoneal fluid was collected by gravity flow into tubes containing potassium-EDTA for cell count and cytologic evaluation and lithium heparin for biochemical analysis. Blood samples were collected via jugular venipuncture into heparinized tubes at the same time. Cytologic components were quantified. Fluid pH and concentrations of total carbon dioxide, sodium, potassium, chloride, lactate, and glucose were compared between peritoneal fluid and venous blood.

Results—All but 3 camelids had peritoneal fluid cell counts of < 3,000 nucleated cells/μL, with < 2,000 neutrophils/μL and < 1,040 large mononuclear cells/μL. All but 1 had peritoneal fluid protein concentrations of ≥ 2.5 g/dL. Peritoneal fluid of camelids generally contained slightly less glucose, lactate, and sodium and roughly equal concentrations of potassium and chloride as venous blood.

Conclusions and Clinical Relevance—Peritoneal fluid was collected safely from healthy camelids. Compared with blood, peritoneal fluid usually had a low cell count and protein concentration, but some individuals had higher values. Electrolyte concentrations resembled those found in blood. High cell counts and protein concentrations found in peritoneal fluid of some healthy camelids may overlap with values found in diseased camelids, complicating interpretation of peritoneal fluid values.

Abstract

Objective—To describe a technique for abdominocentesis in camelids and report peritoneal fluid biochemical and cytologic findings from healthy llamas and alpacas.

Design—Prospective study.

Animals—17 adult llamas and 5 adult alpacas.

Procedures—Right paracostal abdominocentesis was performed. Peritoneal fluid was collected by gravity flow into tubes containing potassium-EDTA for cell count and cytologic evaluation and lithium heparin for biochemical analysis. Blood samples were collected via jugular venipuncture into heparinized tubes at the same time. Cytologic components were quantified. Fluid pH and concentrations of total carbon dioxide, sodium, potassium, chloride, lactate, and glucose were compared between peritoneal fluid and venous blood.

Results—All but 3 camelids had peritoneal fluid cell counts of < 3,000 nucleated cells/μL, with < 2,000 neutrophils/μL and < 1,040 large mononuclear cells/μL. All but 1 had peritoneal fluid protein concentrations of ≥ 2.5 g/dL. Peritoneal fluid of camelids generally contained slightly less glucose, lactate, and sodium and roughly equal concentrations of potassium and chloride as venous blood.

Conclusions and Clinical Relevance—Peritoneal fluid was collected safely from healthy camelids. Compared with blood, peritoneal fluid usually had a low cell count and protein concentration, but some individuals had higher values. Electrolyte concentrations resembled those found in blood. High cell counts and protein concentrations found in peritoneal fluid of some healthy camelids may overlap with values found in diseased camelids, complicating interpretation of peritoneal fluid values.

Contributor Notes

Funded by the Willamette Valley Llama Foundation.

An abstract was presented at the 2007 International Camelid Health Conference, Corvallis, OR.

Address correspondence to Dr. Cebra.
  • 1.

    Cebra CK, Cebra ML, Garry FB, et al. Acute gastrointestinal disease in 27 New World camelids: clinical and surgical findings. Vet Surg 1998;27:112121.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 2.

    Hewson J, Cebra CK. Peritonitis in a llama caused by Streptococcus equi subsp. zooepidemicus. Can Vet J 2001;42:465467.

  • 3.

    Garry F. Clinical pathology of llamas. Vet Clin North Am Food Anim Pract 1989;5:5570.

  • 4.

    Fowler ME. Clinical diagnosis: examination and procedures. In: Fowler ME, ed. Medicine and surgery of South American camelids. Ames, Iowa: Iowa State University Press, 1989;3550.

    • Search Google Scholar
    • Export Citation
  • 5.

    Cebra CK, Heidel JR, Cebra ML, et al. Pathogenesis of Streptococcus zooepidemicus infection after intratracheal inoculation in llamas. Am J Vet Res 2000;61:15251529.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 6.

    Pearson EG, Snyder SP. Pancreatic necrosis in New World camelids: 11 cases (1990–1998). J Am Vet Med Assoc 2000;217:241244.

  • 7.

    Andrews AL, Rawlinson RJ, Raisis AL, et al. Suspected traumatic rupture of the urinary bladder in an alpaca. Aust Vet J 1995;72:7375.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 8.

    Dart AJ, Dart CM, Hodgson DR. Surgical management of a ruptured bladder secondary to a urethral obstruction in an alpaca. Aust Vet J 1997;75:793795.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 9.

    Costarella CE, Anderson DE. Ileocecocolic intussusception in a one-month-old llama. J Am Vet Med Assoc 1999;214:16721673.

  • 10.

    Cebra CK. Biochemical abnormalities in blood and forestomach fluid from llamas with gastrointestinal disease, in Proceedings. 15th Annu Vet Med Forum 1997;447448.

    • Search Google Scholar
    • Export Citation
  • 11.

    Cebra CK. Interpretation of clinical chemistries in llamas, in Proceedings. 23rd Annu Vet Med Forum 2005;272274.

  • 12.

    Cebra CK. Camelid blood test interpretations, in Proceedings. North Am Vet Conf 2006;268271.

  • 13.

    Stewart PA. Modern quantitative acid-base chemistry. Can J Physiol Pharmacol 1983;61:14441461.

  • 14.

    Wellman ML, DiBartola SP, Kohn CW. Applied physiology of body fluids in dogs and cats. In: DiBartola SP, ed. Fluid, electrolyte, and acid-base disorders in small animal practice. 3rd ed. St Louis: Saunders Elsevier, 2006;344.

    • Search Google Scholar
    • Export Citation
  • 15.

    Cebra CK, Tornquist SJ, Vap LM, et al. A comparison of coulometric titration and potentiometric determination of chloride concentration in rumen fluid. Vet Clin Pathol 2001;30:211213.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 16.

    Saulez MN, Cebra CK, Dailey M. Comparative biochemical analyses of venous blood and peritoneal fluid from horses with colic using a portable analyser and an in-house analyser. Vet Rec 2005;157:217223.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 17.

    Latson KM, Nieto JE, Beldomenico PM, et al. Evaluation of peritoneal fluid lactate as a marker of intestinal ischaemia in equine colic. Equine Vet J 2005;37:342346.

    • Search Google Scholar
    • Export Citation
  • 18.

    Noh SM. Measurement of peritoneal fluid pH in patients with non-serosal invasive gastric cancer. Yonsei Med J 2003;44:4548.

  • 19.

    Wilkes P. Hypoproteinemia, strong-ion difference, and acid-base status in critically ill patients. J Appl Physiol 1998;84:17401748.

  • 20.

    McAuliffe JJ, Lind LJ, Leith DE, et al. Hypoproteinemic alkalosis. Am J Med 1986;81:8690.

  • 21.

    Gentry A, Clutton-Brock J, Groves CP. The naming of wild animal species and their domestic derivatives. J Archaeol Sci 2004;31:645651.

  • 22.

    Kadwell M, Fernandez M, Stanley HF, et al. Genetic analysis reveals the wild ancestors of the llama and the alpaca. Proc Biol Sci 2001;268:25752584.

    • Crossref
    • Search Google Scholar
    • Export Citation

Advertisement