Objective—To determine frequency with which
Staphylococcus schleiferi could be isolated from dogs
with pyoderma and antimicrobial susceptibility patterns
of isolates that were obtained.
Animals—54 dogs with a first (n = 14) or recurrent
(40) episode of pyoderma.
Procedure—Specimens were obtained and submitted
for bacterial culture. Isolates were identified as S
schleiferi on the basis of growth and biochemical
characteristics. Two isolates were submitted for DNA
sequencing to confirm identification. Methicillin susceptibility
was determined by means of disk diffusion
with oxacillin-impregnated disks.
Results—3 of 14 dogs examined because of a first
episode of pyoderma and 12 of 40 dogs examined
because of a recurrent episode of pyoderma were
receiving antimicrobials at the time of specimen collection.
Staphylococcus schleiferi was not isolated
from any dog with first-time pyoderma but was isolated
from 5 dogs with recurrent pyoderma that were
not receiving antimicrobials at the time of specimen
collection and 10 dogs with recurrent pyoderma that
were receiving antimicrobials. Nine isolates were
identified as S schleiferi subsp schleiferi, and 6 were
identified as S schleiferi subsp coagulans. All S
schleiferi subsp schleiferi isolates were resistant to
methicillin, but only 2 S schleiferi subsp coagulans
isolates were. Two methicillin-resistant isolates were
also resistant to fluoroquinolones, and 1 isolate had
intermediate susceptibility to fluoroquinolones.
Conclusions and Clinical Relevance—Results suggest
that S schleiferi subsp schleiferi and S schleiferi
subsp coagulans may be isolated from dogs with recurrent
pyoderma. Although isolates from dogs with pyoderma
were frequently resistant to methicillin, multiple
drug resistance was uncommon. (J Am Vet Med Assoc