Objective—To determine the prevalence of bacterial
colonization of IV catheters among young dogs suspected
to have parvoviral enteritis, to identify the
organisms responsible for catheter colonization, and
to determine the antimicrobial susceptibility of organisms
that were obtained.
Procedure—Catheters were aseptically removed
when fluid therapy was discontinued, the catheter
was replaced, or the dog died. The distal tip of the
catheter was cut off, split open, and vortexed with
sterile saline (0.9% NaCl) solution. The saline solution
was plated on culture plates, which were then incubated
and examined for bacterial growth every 24
hours for 72 hours. All bacteria cultured were identified,
and antimicrobial susceptibility was determined.
Results—Bacteria were isolated from 22 catheters.
Most bacteria that were isolated were of gastrointestinal
tract or environmental origin (Serratia odorifera, S
liquefaciens, S marcescens, Acinobacter anitratus,
Citrobacter freundii, Klebsiella pneumoniae, K oxytoca,
Escherichia coli, Enterobacter spp). Only 2 gram-positive
organisms were isolated (Staphylococcus intermedius
and Streptococcus spp). High percentages of
organisms were resistant to penicillin, lincomycin,
cloxacillin, erythromycin, and cephalexin. Percentages
of organisms resistant to amikacin, enrofloxacin, chloramphenicol,
potentiated sulfonamides, and amoxicillin-clavulanic acid were low.
Conclusions and Clinical Relevance—Results suggest
that IV catheters may be colonized with bacteria
in 22% of young dogs suspected to have parvovirus
infection. (J Am Vet Med Assoc 2002;220:1321–1324).