The bacteriome of the oral cavity in healthy dogs and dogs with periodontal disease

Brook A. Niemiec Veterinary Dental Specialties and Oral Surgery, San Diego, CA

Search for other papers by Brook A. Niemiec in
Current site
Google Scholar
PubMed
Close
 DVM
,
Jerzy Gawor Veterinary Clinic Arka, Krakow, Poland

Search for other papers by Jerzy Gawor in
Current site
Google Scholar
PubMed
Close
 DVM, PhD
,
Shuiquan Tang MiDOG LLC, Tustin, CA
Zymo Research Corp., Irvine, CA

Search for other papers by Shuiquan Tang in
Current site
Google Scholar
PubMed
Close
 PhD
,
Aishani Prem MiDOG LLC, Tustin, CA
Zymo Research Corp., Irvine, CA

Search for other papers by Aishani Prem in
Current site
Google Scholar
PubMed
Close
 MSC
, and
Janina A. Krumbeck MiDOG LLC, Tustin, CA
Zymo Research Corp., Irvine, CA

Search for other papers by Janina A. Krumbeck in
Current site
Google Scholar
PubMed
Close
 PhD

Abstract

OBJECTIVE

To compare the bacteriome of the oral cavity in healthy dogs and dogs with various stages of periodontal disease.

ANIMALS

Dogs without periodontal disease (n = 12) or with mild (10), moderate (19), or severe (10) periodontal disease.

PROCEDURES

The maxillary arcade of each dog was sampled with a sterile swab, and swabs were submitted for next-generation DNA sequencing targeting the V1–V3 region of the 16S rRNA gene.

RESULTS

714 bacterial species from 177 families were identified. The 3 most frequently found bacterial species were Actinomyces sp (48/51 samples), Porphyromonas cangingivalis (47/51 samples), and a Campylobacter sp (48/51 samples). The most abundant species were P cangingivalis, Porphyromonas gulae, and an undefined Porphyromonas sp. Porphyromonas cangingivalis and Campylobacter sp were part of the core microbiome shared among the 4 groups, and P gulae, which was significantly enriched in dogs with severe periodontal disease, was part of the core microbiome shared between all groups except dogs without periodontal disease. Christensenellaceae sp, Bacteroidales sp, Family XIII sp, Methanobrevibacter oralis, Peptostreptococcus canis, and Tannerella sp formed a unique core microbiome in dogs with severe periodontal disease.

CONCLUSIONS AND CLINICAL RELEVANCE

Results highlighted that in dogs, potential pathogens can be common members of the oral cavity bacteriome in the absence of disease, and changes in the relative abundance of certain members of the bacteriome can be associated with severity of periodontal disease. Future studies may aim to determine whether these changes are the cause or result of periodontal disease or the host immune response.

Abstract

OBJECTIVE

To compare the bacteriome of the oral cavity in healthy dogs and dogs with various stages of periodontal disease.

ANIMALS

Dogs without periodontal disease (n = 12) or with mild (10), moderate (19), or severe (10) periodontal disease.

PROCEDURES

The maxillary arcade of each dog was sampled with a sterile swab, and swabs were submitted for next-generation DNA sequencing targeting the V1–V3 region of the 16S rRNA gene.

RESULTS

714 bacterial species from 177 families were identified. The 3 most frequently found bacterial species were Actinomyces sp (48/51 samples), Porphyromonas cangingivalis (47/51 samples), and a Campylobacter sp (48/51 samples). The most abundant species were P cangingivalis, Porphyromonas gulae, and an undefined Porphyromonas sp. Porphyromonas cangingivalis and Campylobacter sp were part of the core microbiome shared among the 4 groups, and P gulae, which was significantly enriched in dogs with severe periodontal disease, was part of the core microbiome shared between all groups except dogs without periodontal disease. Christensenellaceae sp, Bacteroidales sp, Family XIII sp, Methanobrevibacter oralis, Peptostreptococcus canis, and Tannerella sp formed a unique core microbiome in dogs with severe periodontal disease.

CONCLUSIONS AND CLINICAL RELEVANCE

Results highlighted that in dogs, potential pathogens can be common members of the oral cavity bacteriome in the absence of disease, and changes in the relative abundance of certain members of the bacteriome can be associated with severity of periodontal disease. Future studies may aim to determine whether these changes are the cause or result of periodontal disease or the host immune response.

Supplementary Materials

    • Supplementary Figure S1 (PDF 501 KB)
    • Supplementary Figure S2 (PDF 502 KB)
    • Supplementary Figure S3 (PDF 407 KB)
    • Supplementary Table S1 (PDF 104 KB)
    • Supplementary Table S2 (PDF 166 KB)
All Time Past Year Past 30 Days
Abstract Views 975 0 0
Full Text Views 8099 6106 1863
PDF Downloads 3845 1947 190
Advertisement