• 1.

    Couëtil LL, Cardwell JM, Gerber V, et al. Inflammatory airway disease of horses-revised consensus statement. J Vet Intern Med 2016;30:503515.

  • 2.

    Pirie RS. Recurrent airway obstruction: a review. Equine Vet J 2014;46:276288.

  • 3.

    Bullone M, Lavoie JP. Science-in-brief: equine asthma diagnosis: beyond bronchoalveolar lavage cytology. Equine Vet J 2017;49:263265.

  • 4.

    Barton AK, Gehlen H. Pulmonary remodeling in equine asthma: what do we know about mediators of inflammation in the horse? Mediators Inflamm 2016;2016:5693205.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 5.

    Harris N, Kunicka J, Kratz A. The ADVIA 2120 hematology system: flow cytometry-based analysis of blood and body fluids in the routine hematology laboratory. Lab Hematol 2005;11:4761.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 6.

    Yonezawa K, Morimoto N, Matsui K, et al. Significance of the neutrophil myeloperoxidase index in patients with atherosclerotic diseases. Kobe J Med Sci 2013;58:E128E137.

    • Search Google Scholar
    • Export Citation
  • 7.

    Hooijberg EH, van den Hoven R, Tichy A, et al. Diagnostic and predictive capability of routine laboratory tests for the diagnosis and staging of equine inflammatory disease. J Vet Intern Med 2014;28:15871593.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 8.

    Leckie MJ, Gomma AH, Purcell IF, et al. Automated quantitation of peripheral blood neutrophil activation in patients with myocardial ischaemia. Int J Cardiol 2004;95:307313.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 9.

    Yonezawa K, Horie O, Yoshioka A, et al. Association between the neutrophil myeloperoxidase index and subsets of bacterial infections. Int J Lab Hematol 2010;32:598605.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 10.

    Gulley ML, Bentley SA, Ross DW. Neutrophil myeloperoxidase measurement uncovers masked megaloblastic anemia. Blood 1990;76:10041007.

  • 11.

    Krause JR, Costello RT, Krause J, et al. Use of the Technicon H-1 in the characterization of leukemias. Arch Pathol Lab Med 1988;112:889894.

    • Search Google Scholar
    • Export Citation
  • 12.

    Arellano-Rodrigo E, Alvarez-Larran A, Reverter JC, et al. Automated assessment of the neutrophil and platelet activation status in patients with essential thrombocythemia. Platelets 2012;23:336343.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 13.

    Allen RC, Stephens JT Jr. Myeloperoxidase selectively binds and selectively kills microbes. Infect Immun 2011;79:474485.

  • 14.

    Leckie MJ, Bryan SA, Khan J, et al. Automated quantitation of circulating neutrophil and eosinophil activation in asthmatic patients. Thorax 2000;55:471477.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 15.

    Klenner S, Richartz J, Bauer N, et al. Myeloperoxidase deficiency in dogs observed with the ADVIA(R)120. A retrospective study. Tierarztl Prax Ausg K Kleintiere Heimtiere 2010;38:139146.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 16.

    Schwarz BC, van den Hoven R, Schwendenwein I. Diagnostic value of the neutrophil myeloperoxidase index in horses with systemic inflammation. Vet J 2012;191:7278.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 17.

    Piviani M, Segura D, Monreal L, et al. Neutrophilic myeloperoxidase index and mean light absorbance in neonatal septic and nonseptic foals. Vet Clin Pathol 2011;40:340344.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 18.

    Lavoie-Lamoureux A, Leclere M, Lemos K, et al. Markers of systemic inflammation in horses with heaves. J Vet Intern Med 2012;26:14191426.

  • 19.

    Bullone M, de Lagarde M, Vargas A, et al. Serum surfactant protein d and haptoglobin as potential biomarkers for inflammatory airway disease in horses. J Vet Intern Med 2015;29:17071711.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 20.

    Gy C, Leclere M, Vargas A, et al. Investigation of blood bio-markers for the diagnosis of mild to moderate asthma in horses (Erratum published in J Vet Intern Med 2019;33:2548– 2550). J Vet Intern Med 2019;33:17891795.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 21.

    Vargas A, Boivin R, Cano P, et al. Neutrophil extracellular traps are downregulated by glucocorticosteroids in lungs in an equine model of asthma. Respir Res 2017;18:207.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 22.

    Lavoie J-P, Bullone M, Rodrigues N, et al. Effect of different doses of inhaled ciclesonide on lung function, clinical signs related to airflow limitation and serum cortisol levels in horses with experimentally induced mild to severe airway obstruction. Equine Vet J 2019;51:779786.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 23.

    van Erck E, Votion D, Art T, et al. Measurement of respiratory function by impulse oscillometry in horses. Equine Vet J 2004;36:2128.

  • 24.

    Jean D, Vrins A, Beauchamp G, et al. Evaluation of variations in bronchoalveolar lavage fluid in horses with recurrent airway obstruction. Am J Vet Res 2011;72:838842.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 25.

    Linderkamp O, Ruef P, Brenner B, et al. Passive deformability of mature, immature, and active neutrophils in healthy and septicemic neonates. Pediatr Res 1998;44:946950.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 26.

    Teran LM, Campos MG, Begishvilli BT, et al. Identification of neutrophil chemotactic factors in bronchoalveolar lavage fluid of asthmatic patients. Clin Exp Allergy 1997;27:396405.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 27.

    Shute JK, Vrugt B, Lindley IJ, et al. Free and complexed interleukin-8 in blood and bronchial mucosa in asthma. Am J Respir Crit Care Med 1997;155:18771883.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 28.

    Lavoie-Lamoureux A, Moran K, Beauchamp G, et al. IL-4 activates equine neutrophils and induces a mixed inflammatory cytokine expression profile with enhanced neutrophil chemotactic mediator release ex vivo. Am J Physiol Lung Cell Mol Physiol 2010;299:L472L482.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 29.

    Lavoie-Lamoureux A, Beauchamp G, Quessy S, et al. Systemic inflammation and priming of peripheral blood leukocytes persist during clinical remission in horses with heaves. Vet Immunol Immunopathol 2012;146:3545.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 30.

    Beekman L, Tohver T, Leguillette R. Comparison of cytokine mRNA expression in the bronchoalveolar lavage fluid of horses with inflammatory airway disease and bronchoalveolar lavage mastocytosis or neutrophilia using REST software analysis. J Vet Intern Med 2012;26:153161.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 31.

    Ziaei JE, Dastgiri S. Role of myeloperoxidase index in differentiation of megaloblastic and aplastic anemia. Indian J Med Sci 2004;58:345348.

    • Search Google Scholar
    • Export Citation
  • 32.

    Buffon A, Biasucci LM, Liuzzo G, et al. Widespread coronary inflammation in unstable angina. N Engl J Med 2002;347:512.

  • 33.

    Art T, Franck T, Lekeux P, et al. Myeloperoxidase concentration in bronchoalveolar lavage fluid from healthy horses and those with recurrent airway obstruction. Can J Vet Res 2006;70:291296.

    • Search Google Scholar
    • Export Citation
  • 34.

    Leclere M, Lavoie-Lamoureux A, Lavoie JP. Heaves, an asthma-like disease of horses. Respirology 2011;16:10271046.

  • 35.

    Lilliehöök I, Tvedten HW, Bröjer J, et al. Time-related changes in equine neutrophils after experimental endotoxemia: myeloperoxidase staining, size, and numbers. Vet Clin Pathol 2016;45:6672.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 36.

    Coates TD, Wolach B, Tzeng DY, et al. The mechanism of action of the antiinflammatory agents dexamethasone and Auranofin in human polymorphonuclear leukocytes. Blood 1983;62:10701077.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 37.

    Lecoq L, Vincent P, Lavoie-Lamoureux A, et al. Genomic and non-genomic effects of dexamethasone on equine peripheral blood neutrophils. Vet Immunol Immunopathol 2009;128:126131.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 38.

    Picandet V, Leguillette R, Lavoie JP. Comparison of efficacy and tolerability of isoflupredone and dexamethasone in the treatment of horses affected with recurrent airway obstruction (‘heaves’). Equine Vet J 2003;35:419424.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 39.

    Leclere M, Lefebvre-Lavoie J, Beauchamp G, et al. Efficacy of oral prednisolone and dexamethasone in horses with recurrent airway obstruction in the presence of continuous antigen exposure. Equine Vet J 2010;42:316321.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 40.

    Cohn LA. The influence of corticosteroids on host defense mechanisms. J Vet Intern Med 1991;5:95104.

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Retrospective investigation of automated hematology analyzer–determined indicators of neutrophil activation in blood samples from horses with asthma

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  • 1 From the Departments of Clinical Sciences, University of Montreal, St-Hyacinthe, QC J2S 2M2, Canada.
  • | 2 From the Departments of Pathology and Microbiology, Faculty of Veterinary Medicine, University of Montreal, St-Hyacinthe, QC J2S 2M2, Canada.

Abstract

OBJECTIVE

To investigate indicators of neutrophil activation in the blood of healthy and asthma-affected horses and assess associations between corticosteroid treatment and these variables.

ANIMALS

48 horses (14 with severe equine asthma [SEA], 21 with mild to moderate equine asthma [MEA], and 13 healthy controls).

PROCEDURES

In a 3-part retrospective study, hematology analyzer data for horses included in previous studies were reviewed. Neutrophil size, neutrophil light absorbance (NLA), and myeloperoxidase (MPO) index were recorded. Data for each variable were compared among groups for the entire study sample (part 1). Changes in each variable were assessed for one subset of horses (5 SEA-affected and 6 controls) after treatment for 2 weeks with dexamethasone (0.06 mg/kg, PO, q 24 h; part 2) and for another subset (8 SEA-affected horses) after the same treatment and after a 1-week post-treatment washout period (part 3).

RESULTS

All 3 variables were significantly greater for the SEA group, compared with the MEA and control groups in part 1. Following dexamethasone treatment, the control- and SEA-group NLA and MPO index significantly decreased and SEA-group neutrophil size significantly decreased in part 2; immediate posttreatment results for SEA-affected horses were similar in part 3, with significantly increased neutrophil size and nonsignificant increases in NLA and MPO index following washout.

CONCLUSIONS AND CLINICAL RELEVANCE

Results suggested horses with exacerbated SEA have larger neutrophils that contain more MPO, compared with neutrophils of MEA-affected and healthy control horses. The clinical value of these variables for the diagnosis of equine asthma was deemed limited owing to data overlap among groups. (Am J Vet Res 2021;82:737–745)

Abstract

OBJECTIVE

To investigate indicators of neutrophil activation in the blood of healthy and asthma-affected horses and assess associations between corticosteroid treatment and these variables.

ANIMALS

48 horses (14 with severe equine asthma [SEA], 21 with mild to moderate equine asthma [MEA], and 13 healthy controls).

PROCEDURES

In a 3-part retrospective study, hematology analyzer data for horses included in previous studies were reviewed. Neutrophil size, neutrophil light absorbance (NLA), and myeloperoxidase (MPO) index were recorded. Data for each variable were compared among groups for the entire study sample (part 1). Changes in each variable were assessed for one subset of horses (5 SEA-affected and 6 controls) after treatment for 2 weeks with dexamethasone (0.06 mg/kg, PO, q 24 h; part 2) and for another subset (8 SEA-affected horses) after the same treatment and after a 1-week post-treatment washout period (part 3).

RESULTS

All 3 variables were significantly greater for the SEA group, compared with the MEA and control groups in part 1. Following dexamethasone treatment, the control- and SEA-group NLA and MPO index significantly decreased and SEA-group neutrophil size significantly decreased in part 2; immediate posttreatment results for SEA-affected horses were similar in part 3, with significantly increased neutrophil size and nonsignificant increases in NLA and MPO index following washout.

CONCLUSIONS AND CLINICAL RELEVANCE

Results suggested horses with exacerbated SEA have larger neutrophils that contain more MPO, compared with neutrophils of MEA-affected and healthy control horses. The clinical value of these variables for the diagnosis of equine asthma was deemed limited owing to data overlap among groups. (Am J Vet Res 2021;82:737–745)

Supplementary Materials

    • Supplementary Table S1 (PDF 87 kb)

Contributor Notes

Address correspondence to Dr. Herteman (nherteman. dacvimla@gmail.com).