In vitro effects of epinephrine, norepinephrine, and dobutamine on lipopolysaccharide-stimulated production of tumor necrosis factor-α, interleukin-6, and interleukin-10 in blood from healthy dogs

Kristen M. Merrill Comparative Internal Medicine Laboratory, Veterinary Health Center, College of Veterinary Medicine, University of Missouri, Columbia, MO 65211.

Search for other papers by Kristen M. Merrill in
Current site
Google Scholar
PubMed
Close
 DVM
,
Matthew B. Hull Comparative Internal Medicine Laboratory, Veterinary Health Center, College of Veterinary Medicine, University of Missouri, Columbia, MO 65211.

Search for other papers by Matthew B. Hull in
Current site
Google Scholar
PubMed
Close
 DVM
,
Aaron Stoker Thompson Laboratory for Regenerative Orthopaedics, School of Medicine, University of Missouri, Columbia, MO 65211.

Search for other papers by Aaron Stoker in
Current site
Google Scholar
PubMed
Close
 PhD
, and
Amy E. DeClue Comparative Internal Medicine Laboratory, Veterinary Health Center, College of Veterinary Medicine, University of Missouri, Columbia, MO 65211.

Search for other papers by Amy E. DeClue in
Current site
Google Scholar
PubMed
Close
 DVM, MS

Click on author name to view affiliation information

Abstract

OBJECTIVE

To determine the in vitro effects of epinephrine, norepinephrine, and dobutamine on lipopolysaccharide (LPS)-stimulated production of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-10 (IL-10) in blood from healthy dogs.

SAMPLES

Blood samples from 9 healthy dogs.

PROCEDURES

Blood samples were incubated with LPS from Escherichia coli O127:B8 or PBSS (control) for 1 hour. Afterward, the samples were incubated with 10μM epinephrine, norepinephrine, or dobutamine or with saline (0.9% NaCl) solution (control) for 23 hours. Leukocyte viability was assessed by use of trypan-blue exclusion in blood from 2 dogs to ensure cell viability was not altered by the catecholamines. Tumor necrosis factor-α, IL-6, and IL-10 concentrations were measured in the supernatant in duplicate with a canine-specific multiplex bead-based assay. Blood samples from 2 dogs were used to create dose-response curves to evaluate whether the observed cytokine modulation was dependent on catecholamine concentration.

RESULTS

Incubation of blood with epinephrine and norepinephrine significantly increased LPS-stimulated production of IL-10, compared with the control. Epinephrine and norepinephrine significantly decreased LPS-stimulated production of TNF-α, compared with the control. Epinephrine and norepinephrine did not significantly alter LPS-stimulated production of IL-6. Dobutamine did not alter catecholamine production.

CONCLUSIONS AND CLINICAL RELEVANCE

Epinephrine and norepinephrine, but not dobutamine, had immunomodulatory effects on LPS-stimulated TNF-α and IL-10 production in blood from healthy dogs in this in vitro model of sepsis. Data suggested that dobutamine may have immune system-sparing effects in dogs with sepsis.

Abstract

OBJECTIVE

To determine the in vitro effects of epinephrine, norepinephrine, and dobutamine on lipopolysaccharide (LPS)-stimulated production of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-10 (IL-10) in blood from healthy dogs.

SAMPLES

Blood samples from 9 healthy dogs.

PROCEDURES

Blood samples were incubated with LPS from Escherichia coli O127:B8 or PBSS (control) for 1 hour. Afterward, the samples were incubated with 10μM epinephrine, norepinephrine, or dobutamine or with saline (0.9% NaCl) solution (control) for 23 hours. Leukocyte viability was assessed by use of trypan-blue exclusion in blood from 2 dogs to ensure cell viability was not altered by the catecholamines. Tumor necrosis factor-α, IL-6, and IL-10 concentrations were measured in the supernatant in duplicate with a canine-specific multiplex bead-based assay. Blood samples from 2 dogs were used to create dose-response curves to evaluate whether the observed cytokine modulation was dependent on catecholamine concentration.

RESULTS

Incubation of blood with epinephrine and norepinephrine significantly increased LPS-stimulated production of IL-10, compared with the control. Epinephrine and norepinephrine significantly decreased LPS-stimulated production of TNF-α, compared with the control. Epinephrine and norepinephrine did not significantly alter LPS-stimulated production of IL-6. Dobutamine did not alter catecholamine production.

CONCLUSIONS AND CLINICAL RELEVANCE

Epinephrine and norepinephrine, but not dobutamine, had immunomodulatory effects on LPS-stimulated TNF-α and IL-10 production in blood from healthy dogs in this in vitro model of sepsis. Data suggested that dobutamine may have immune system-sparing effects in dogs with sepsis.

All Time Past Year Past 30 Days
Abstract Views 384 0 0
Full Text Views 1641 989 104
PDF Downloads 708 254 26
Advertisement