Safety and efficacy of cold atmospheric plasma for the sterilization of a Pasteurella multocida–contaminated subcutaneously implanted foreign body in rabbits
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Abstract
OBJECTIVE
To determine whether a stainless steel implant sterilized with a novel cold atmospheric plasma sterilization (CAPS) device adversely affects local tissues in rabbits and whether CAPS was as effective as steam sterilization with an autoclave to inactivate Pasteurella multocida.
ANIMALS
31 healthy New Zealand White rabbits.
PROCEDURES
Steam-autoclaved stainless steel implants inoculated with P multocida underwent a second steam autoclave sterilization (AIA) or CAPS (AICAPS). One AIA implant and 3 AICAPS implants were randomly placed subcutaneously at 4 sites in 21 rabbits (84 implants). These rabbits were monitored daily for 5 days for evidence of systemic illness and local tissue reactions at the implantation sites and then euthanized. Samples were taken from each implant site for bacterial culture and histologic examination.
RESULTS
Cultures of samples obtained from all sites were negative for bacterial growth. No significant difference was observed in mean skin thickness or erythema between AIA and AICAPS implant sites on any observed day. Also, individual histologic grades for the epidermis, dermis, subcutis, and muscle and total histologic grade were not significantly different between AIA and AICAPS implant sites.
CONCLUSIONS AND CLINICAL RELEVANCE
Cold atmospheric plasma sterilization was noninferior to steam sterilization of P multocida–contaminated stainless steel implants in the rabbits in the present study. However, studies of the efficacy of CAPS for inactivation of other important bacteria are needed.
Supplementary Materials
- Supplementary Figure S1 (PDF 129 KB)
- Supplementary Figure S2 (PDF 210 KB)
- Supplementary Table S1 (PDF 124 KB)
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- PubMed Citation
- Article by Haileigh K. Avellar
- Article by Megan R. Williams
- Article by João Brandão
- Article by Sai Narayanan
- Article by Akhilesh Ramachandran
- Article by Todd C. Holbrook
- Article by Mike J. Schoonover
- Article by Keith L. Bailey
- Article by Mark E. Payton
- Article by Kedar Kamlakant Pai
- Article by Chris T. Timmons
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