• 1. Bertram CA, Muller K, Klopfleisch R. Genital tract pathology in female pet rabbits (Oryctolagus cuniculus): a retrospective study of 854 necropsy examinations and 152 biopsy samples. J Comp Pathol 2018;164:1726.

    • Search Google Scholar
    • Export Citation
  • 2. Künzel F, Grinninger P, Shibly S, et al. Uterine disorders in 50 pet rabbits. J Am Anim Hosp Assoc 2015;51:814.

  • 3. Walter B, Poth T, Bohmer E, et al. Uterine disorders in 59 rabbits. Vet Rec 2010;166:230233.

  • 4. Saito K, Nakanishi M, Hasegawa A. Uterine disorders diagnosed by ventrotomy in 47 rabbits. J Vet Med Sci 2002;64:495497.

  • 5. Varga M. Textbook of rabbit medicine. 2nd ed. Edinburgh: Butterworth-Heinemann, 2014;430432.

  • 6. Brodbelt DC, Blissitt KJ, Hammond RA, et al. The risk of death: the confidential enquiry into perioperative small animal fatalities. Vet Anaesth Analg 2008;35:365373.

    • Search Google Scholar
    • Export Citation
  • 7. Self I. A basic approach to small mammal anaesthesia. Ir Vet J 2007;60:94100.

  • 8. Barter LS. Rabbit analgesia. Vet Clin North Am Exot Anim Pract 2011;14:93104.

  • 9. Flecknell PA, Liles JH. Assessment of the analgesic action of opioid agonist-antagonists in the rabbit. J Assoc Vet Anaesth G B Ir 1990;17:2429.

    • Search Google Scholar
    • Export Citation
  • 10. Flecknell PA, Roughan JV. Assessing pain in animals—putting research into practice. Anim Welf 2004;13(suppl 1):S71-S75.

  • 11. Coleman KA, Monnet E, Johnston MS. Single port laparoscopic-assisted ovariohysterectomy in 3 rabbits. J Exot Pet Med 2018;27:2124.

  • 12. McCracken BD, Sasha H, Stewart DR, et al. Comparison of isobaric and insufflated laparoscopy-assisted ovariohysterectomy in the common rabbit (Oryctolagus cuniculus). J Exot Pet Med 2019;28:193204.

    • Search Google Scholar
    • Export Citation
  • 13. Chaniotakis I, Antoniou E, Kostomitsopoulos N, et al. Stress response to ovariohysterectomy in rabbits: role of anaesthesia and surgery. J Obstet Gynaecol 2018;38:697701.

    • Search Google Scholar
    • Export Citation
  • 14. Divers SJ. Clinical technique: endoscopic oophorectomy in the rabbit (Oryctolagus cuniculus): the future of preventative sterilizations. J Exot Pet Med 2010;19:231239.

    • Search Google Scholar
    • Export Citation
  • 15. Karande VC. LigaSure 5-mm blunt tip laparoscopic instrument. J Obstet Gynaecol India 2015;65:350352.

  • 16. Mathonnet O. LigaSure Atias instrument. Minim Invasive Ther Allied Technol 2002;11:237242.

  • 17. Guzelburc V, Baran C, Yafi FA, et al. Vasectomy with vessel sealing device: comparison of different diameters. Int J Impot Res 2019;31:2024.

    • Search Google Scholar
    • Export Citation
  • 18. Moreno-Sanz C, Picazo-Yeste J, Seoane-Gonzáles J, et al. Division of the small bowel with the LigaSure Atlas device during the right laparoscopic colectomy. J Laparoendosc Adv Surg Tech A 2008;18:99101.

    • Search Google Scholar
    • Export Citation
  • 19. Santini M, Fiorelli A, Messina G, et al. The feasibility of Liga Sure to create intestinal anastomosis: results of ex vivo study. Surg Innov 2015;22:266273.

    • Search Google Scholar
    • Export Citation
  • 20. Schulze S, Damgaard B, Jorgensen LN, et al. Cystic duct closure by sealing with bipolar electrocoagulation. JSLS 2010;14:2022.

  • 21. Shamiyeh A, Vattay P, Tulipan L, et al. Closure of the cystic duct during laparoscopic cholecystectomy with a new feedback-controlled bipolar sealing system in case of biliary obstruction—an experimental study in pigs. Hepatogastroenterology 2004;51:931933.

    • Search Google Scholar
    • Export Citation
  • 22. Sorgato N, Bernante P, Pelizzo MR. Application of the LigaSure tissue sealing system to intestinal resection. Experimental and clinical trial. Ann Ital Chir 2008;79:383388.

    • Search Google Scholar
    • Export Citation
  • 23. Yekeler E, Ulutas H, Becerik C, et al. The use of the LigaSure in esophagectomy. Interact Cardiovasc Thorac Surg 2010;11:1014.

  • 24. Lacin T, Batirel HF, Ozer K, et al. Safety of a thermal vessel sealer on main pulmonary vessels. Eur J Cardiothorac Surg 2007;31:482485.

    • Search Google Scholar
    • Export Citation
  • 25. Brückner M, Heblinski N, Henrich M. Use of a novel vessel-sealing device for peripheral lung biopsy and lung lobectomy in a cadaveric model. J Small Anim Pract 2019;60:411416.

    • Search Google Scholar
    • Export Citation
  • 26. Barrera JS, Monnet E. Effectiveness of a bipolar vessel sealant device for sealing uterine horns and bodies from dogs. Am J Vet Res 2012;73:302305.

    • Search Google Scholar
    • Export Citation
  • 27. Mayhew PD, Brown DC. Comparison of three techniques for ovarian pedicle hemostasis during laparoscopic-assisted ovariohysterectomy. Vet Surg 2007;36:541547.

    • Search Google Scholar
    • Export Citation
  • 28. Latimer CA, Nelson M, Moore CM, et al. Bioengineering/nanomedicine: effect of collagen and elastin content on the burst pressure of human blood vessel seals formed with a bipolar tissue sealing system. J Surg Res 2014;186:7380.

    • Search Google Scholar
    • Export Citation
  • 29. Sindram D, Martin K, Meadows JP, et al. Collagen-elastin ratio predicts burst pressure of arterial seals created using a bipolar vessel sealing device in a porcine model. Surg Endosc 2011;25:26042612.

    • Search Google Scholar
    • Export Citation
  • 30. Karsdon J, Garfield RE, Shi SQ, et al. Electrical inhibition of preterm birth: inhibition of uterine contractility in the rabbit and pup births in the rat. Am J Obstet Gynecol 2005;193:19861993.

    • Search Google Scholar
    • Export Citation
  • 31. Hothorn T, Zeileis A, Siebold H. model4you: an R package for personalised treatment effect estimation. J Open Res Softw 2019;7:17.

  • 32. Dyce KM, Sack WO, Wensing CJG. Textbook of veterinary anatomy. St Louis: Elsevier, 1987;459463.

  • 33. O'Malley B. Clinical anatomy and physiology of exotic species: structure and function of mammals, birds, reptiles, and amphibians. Edinburgh: Elsevier, 2005;190191.

    • Search Google Scholar
    • Export Citation

Advertisement

Comparison of the use of a vessel-sealing device versus ligatures for occlusion of uterine tissues during ovariohysterectomy or ovariectomy in rabbits (Oryctolagus cuniculus)

Euan J. McLean DVM1, Andrew P. Woodward BVSC, PHD2, and Stewart D. Ryan BVSC, MS1
View More View Less
  • 1 1Translational Research and Animal Clinical Trial Study (TRACTS) Group, University of Melbourne, Werribee, VIC 3052, Australia.
  • | 2 2Faculty of Veterinary and Agricultural Sciences (Woodward), University of Melbourne, Werribee, VIC 3052, Australia.

Abstract

OBJECTIVE

To compare the bursting strength of the uterine horns (UHs) and cervical-vestibule junction (CVJs) of rabbits following sealing with a vessel-sealing device (VSD) or encircling ligatures.

SAMPLE

UHs and CVJs collected from 30 rabbit (Oryctolagus cuniculus) cadavers.

PROCEDURES

UHs and CVJs were randomly assigned to sealing with encircling Miller knot ligatures (LIG; n = 10 CVJs and 20 UHs) or a VSD (12 CVJs and 24 UHs). Lumens were infused with saline (0.9% NaCl) solution under pressure until seals burst or to a maximum pressure of 300 mm Hg.

RESULTS

For CVJs, median (range) bursting pressure of the LIG and VSD groups was > 300 mm Hg (224 to > 300 mm Hg) and 35 mm Hg (0 to 60 mm Hg), respectively. Five of 12 CVJs in the VSD group failed at pressures < 33 mm Hg. For UHs, median (range) bursting pressure of the LIG and VSD groups was 255 mm Hg (120 to > 300 mm Hg) and 154 mm Hg (range, 44 to 202 mm Hg), respectively.

CONCLUSIONS AND CLINICAL RELEVANCE

The evaluated VSD was effective in sealing UHs at bursting pressures well in excess of expected physiologic pressures, indicating that the VSD may be useful for ovariectomy procedures in rabbits. However, CVJ seals created with the VSD were ineffective and could potentially burst at low pressures, which could predispose to urine entering the abdomen. Given these results, we do not recommend sealing of the CVJ with a VSD for ovariohysterectomy in rabbits.

Abstract

OBJECTIVE

To compare the bursting strength of the uterine horns (UHs) and cervical-vestibule junction (CVJs) of rabbits following sealing with a vessel-sealing device (VSD) or encircling ligatures.

SAMPLE

UHs and CVJs collected from 30 rabbit (Oryctolagus cuniculus) cadavers.

PROCEDURES

UHs and CVJs were randomly assigned to sealing with encircling Miller knot ligatures (LIG; n = 10 CVJs and 20 UHs) or a VSD (12 CVJs and 24 UHs). Lumens were infused with saline (0.9% NaCl) solution under pressure until seals burst or to a maximum pressure of 300 mm Hg.

RESULTS

For CVJs, median (range) bursting pressure of the LIG and VSD groups was > 300 mm Hg (224 to > 300 mm Hg) and 35 mm Hg (0 to 60 mm Hg), respectively. Five of 12 CVJs in the VSD group failed at pressures < 33 mm Hg. For UHs, median (range) bursting pressure of the LIG and VSD groups was 255 mm Hg (120 to > 300 mm Hg) and 154 mm Hg (range, 44 to 202 mm Hg), respectively.

CONCLUSIONS AND CLINICAL RELEVANCE

The evaluated VSD was effective in sealing UHs at bursting pressures well in excess of expected physiologic pressures, indicating that the VSD may be useful for ovariectomy procedures in rabbits. However, CVJ seals created with the VSD were ineffective and could potentially burst at low pressures, which could predispose to urine entering the abdomen. Given these results, we do not recommend sealing of the CVJ with a VSD for ovariohysterectomy in rabbits.

Contributor Notes

Dr. McLean's present address is Southpaws Specialty Surgery for Animals, Moorabbin, VIC 3189, Australia.

Address correspondence to Dr. Ryan (stewart.ryan@unimelb.edu.au).