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Glomerular filtration rate determined by measuring serum clearance of a single dose of inulin and serum symmetric dimethylarginine concentration in clinically normal cheetahs (Acinonyx jubatus)

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  • 1 1Department of Animal Health, Smithsonian National Zoological Park, Washington, DC 20008.
  • | 2 2Global Health Program, Smithsonian National Zoological Park, Washington, DC 20008.
  • | 3 3Smithsonian Institution, Washington, DC 20013.
  • | 4 4EcoHealth Alliance, New York, NY 10001.
  • | 5 5Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Georgia, Athens, GA 30602.
  • | 6 6White Oak Conservation, Yulee, FL 32097.
  • | 7 7and Cheetah Conservation Fund, Otjiwarongo, Namibia.

Abstract

OBJECTIVE

To establish a reference interval for glomerular filtration rate (GFR) determined by measuring serum clearance of a single IV dose of inulin in clinically normal cheetahs (Acinonyx jubatus) and compare serum symmetric dimethylarginine (SDMA) concentration in cheetahs with GFR.

ANIMALS

33 cheetahs housed at 3 institutions.

PROCEDURES

A single bolus of inulin (3,000 mg/m2) was administered IV, and 5 serial blood samples were collected and analyzed for serum inulin concentration with the anthrone technique. The GFR was estimated with a modified slope-intercept method for the slow component of the serum concentration-versus-time curve. Blood urea nitrogen and serum creatinine concentrations were measured in samples obtained immediately prior to inulin administration, and serum SDMA concentration was measured in stored samples.

RESULTS

Mean ± SD measured GFR was 1.58 ± 0.39 mL/min/kg, and the calculated reference interval was 0.84 to 2.37 mL/min/kg. There were significant negative correlations between GFR and serum creatinine concentration (r = −0.499), BUN concentration (r = −0.592), and age (r = −0.463). Serum SDMA concentration was not significantly correlated with GFR (r = 0.385), BUN concentration (r = −0.281), or serum creatinine concentration (r = 0.165).

CONCLUSIONS AND CLINICAL RELEVANCE

A reference interval for GFR in clinically normal cheetahs was obtained. Further evaluation of animals with renal disease is needed to determine whether measuring serum clearance of a single IV dose of inulin is a reliable diagnostic test for early detection of renal disease in cheetahs.

Contributor Notes

Dr. Sanchez's present address is the Veterinary Medical Center, Oregon Zoo, Portland, OR 97221. Dr. Jones’ present address is the Department of Animal Science, College of Agriculture and Life Sciences, University of Vermont, Burlington, VT 05405.

Address correspondence to Dr. Sanchez (crs0403@gmail.com).