Cover American Journal of Veterinary Research
American Journal of Veterinary Research
ISSN:
0002-9645
Publication Date:
01 Sep 2019

  • 1. Callan MB, Oakley DA, Shofer FS, et al. Canine red blood cell transfusion practice. J Am Anim Hosp Assoc 1996;32:303–311.

  • 2. Hann L, Brown DC, King LG, et al. Effect of duration of packed red blood cell storage on morbidity and mortality in dogs after transfusion: 3,095 cases (2001–2010). J Vet Intern Med 2014;28:1830–1837.

    • Search Google Scholar
    • Export Citation

  • 3. Kerl ME, Hohenhaus AE. Packed red blood cell transfusions in dogs: 131 cases (1989). J Am Vet Med Assoc 1993;202:1495–1499.

  • 4. Lanevschi A, Wardrop KJ. Principles of transfusion medicine in small animals. Can Vet J 2001;42:447–454.

  • 5. Holowaychuk MK, Leader JL, Monteith G. Risk factors for transfusion-associated complications and nonsurvival in dogs receiving packed red blood cell transfusions: 211 cases (2008–2011). J Am Vet Med Assoc 2014;244:431–437.

    • Search Google Scholar
    • Export Citation

  • 6. Hébert PC, Fergusson D, Blajchman MA, et al. Clinical outcomes following institution of the Canadian universal leukoreduction program for red blood cell transfusions. JAMA 2003;289:1941–1949.

    • Search Google Scholar
    • Export Citation

  • 7. Sharma AD, Sreeram G, Erb T, et al. Leukocyte-reduced blood transfusions: perioperative indications, adverse effects, and costs analysis. Anesth Analg 2000;90:1315–1323.

    • Search Google Scholar
    • Export Citation

  • 8. McMichael MA, Smith SA, Galligan A, et al. Effect of leukoreduction on transfusion-induced inflammation in dogs. J Vet Intern Med 2010;24:1131–1137.

    • Search Google Scholar
    • Export Citation

  • 9. Herring JM, Smith SA, McMichael MA, et al. Microparticles in stored canine RBC concentrates. Vet Clin Pathol 2013;42:163–169.

  • 10. Graf C, Raila J, Schweigert FJ, et al. Effect of leukoreduction treatment on vascular endothelial growth factor concentration in stored canine blood transfusion products. Am J Vet Res 2012;73:2001–2006.

    • Search Google Scholar
    • Export Citation

  • 11. Urban R, Couto CG, Iazbik MC. Evaluation of hemostatic activity of canine frozen plasma for transfusion by thromboelastography. J Vet Intern Med 2013;27:964–969.

    • Search Google Scholar
    • Export Citation

  • 12. Alhumaidan HS, Cheves TA, Holme S, et al. The effect of filtration on residual levels of coagulation factors in plasma. Am J Clin Pathol 2013;139:110–116.

    • Search Google Scholar
    • Export Citation

  • 13. Solheim BG, Flesland O, Brosstad F, et al. Improved preservation of coagulation factors after pre-storage leukocyte depletion of whole blood. Transfus Apher Sci 2003;29:133–139.

    • Search Google Scholar
    • Export Citation

  • 14. Kretzschmar E, Kruse F, Greiss O, et al. Effects of extended storage of whole blood before leucocyte depletion on coagulation factors in plasma. Vox Sang 2004;87:156–164.

    • Search Google Scholar
    • Export Citation

  • 15. Cardigan R, Sutherland J, Garwood M, et al. The effect of leucocyte depletion on the quality of fresh-frozen plasma. Br J Haematol 2001;114:233–240.

    • Search Google Scholar
    • Export Citation

  • 16. Wardrop KJ, Brooks MB. Stability of hemostatic proteins in canine fresh frozen plasma units. Vet Clin Pathol 2001;30:91–95.

  • 17. Dumont LJ, Cancelas JA, Maes LA, et al. The bioequivalence of frozen plasma prepared from whole blood held overnight at room temperature compared to fresh-frozen plasma prepared within eight hours of collection. Transfusion 2015;55:476–484.

    • Search Google Scholar
    • Export Citation

  • 18. Stokol T, Brooks MB, Erb HN. Effect of citrate concentration on coagulation test results in dogs. J Am Vet Med Assoc 2000;217:1672–1677.

    • Search Google Scholar
    • Export Citation

  • 19. Clauss A. Gerinnungsphysiologische schnellmethode zur bestimmung des fibrinogens. Acta Haematol 1957;17:237–246.

  • 20. Kisielewicz C, Self IA. Canine and feline blood transfusions: controversies and recent advances in administration practices. Vet Anaesth Analg 2014;41:233–242.

    • Search Google Scholar
    • Export Citation

  • 21. Walton JE, Hale AS, Brooks MB, et al. Coagulation factor and hemostatic protein content of canine plasma after storage of whole blood at ambient temperature. J Vet Intern Med 2014;28:571–575.

    • Search Google Scholar
    • Export Citation

  • 22. Scott E, Puca K, Heraly J, et al. Evaluation and comparison of coagulation factor activity in fresh-frozen plasma and 24-hour plasma at thaw and after 120 hours of 1 to 6°C storage. Transfusion 2009;49:1584–1591.

    • Search Google Scholar
    • Export Citation

  • 23. Williamson LM, Rider JR, Swann ID, et al. Evaluation of plasma and red cells obtained after leucocyte depletion of whole blood. Transfus Med 1999;9:51–61.

    • Search Google Scholar
    • Export Citation

  • 24. Heiden M, Salge U, Henschler R, et al. Plasma quality after whole-blood filtration depends on storage temperature and filter type. Transfus Med 2004;14:297–304.

    • Search Google Scholar
    • Export Citation

  • 25. Runkel S, Bach J, Haubelt H, et al. The impact of two whole blood inline filters on markers of coagulation, complement and cell activation. Vox Sang 2005;88:17–21.

    • Search Google Scholar
    • Export Citation

  • 26. Shiba M, Tadokoro K, Sawanobori M, et al. Activation of the contact system by filtration of platelet concentrates with a negatively charged white cell-removal filter and measurement of venous blood bradykinin level in patients who received filtered platelets. Transfusion 1997;37:457–462.

    • Search Google Scholar
    • Export Citation

  • 27. Lippi G, Salvagno GL, Montagnana M, et al. Quality standards for sample collection in coagulation testing. Semin Thromb Hemost 2012;38:565–575.

    • Search Google Scholar
    • Export Citation

  • 28. Magnette A, Chatelain M, Chatelain B, et al. Pre-analytical issues in the haemostasis laboratory: guidance for the clinical laboratories. Thromb J 2016;14:49.

    • Search Google Scholar
    • Export Citation

  • 29. Brownlee L, Wardrop KJ, Sellon RK, et al. Use of a prestorage leukoreduction filter effectively removes leukocytes from canine whole blood while preserving red blood cell viability. J Vet Intern Med 2000;14:412–417.

    • Search Google Scholar
    • Export Citation

  • 30. Dzik S. Principles of counting low numbers of leukocytes in leukoreduced blood components. Transfus Med Rev 1997;11:44–55.

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Editorial Type:
Hematology

Coagulation factor activity in units of leukoreduced and nonleukoreduced canine fresh-frozen plasma

Michelle L. Foote DVM1, Marjory B. Brooks DVM3, Todd M. Archer DVM, MS1, Robert W. Wills DVM, PhD2, Andrew J. Mackin BVMS, DVSc1, and John M. Thomason DVM, MS1,2,3
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  • 1 1Department of Clinical Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762.
  • | 2 2Department of Pathobiology and Population Medicine, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762.
  • | 3 3Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853.
DOI:
https://doi.org/10.2460/ajvr.80.9.846
Volume/Issue:
Volume 80: Issue 9
Online Publication Date:
01 Sep 2019
Restricted access
Reprints and Permissions Purchase Article

Abstract

OBJECTIVE

To evaluate coagulation factors in units of leukoreduced (LR) and nonleukoreduced (non-LR) canine fresh-frozen plasma (cFFP).

ANIMALS

8 healthy research dogs.

PROCEDURES

In a crossover study, dogs were randomly assigned to 1 of 2 groups from which blood was collected and either did or did not undergo leukoreduction. After a recovery period of ≥ 28 days, the dogs were switched between protocols. After each collection, blood samples were centrifuged, and cFFP was stored frozen for later comparative analysis of coagulation factors, antithrombin, and protein C activities (reported as comparative percentages of the corresponding activities determined in a canine pooled plasma standard); prothrombin and activated partial thromboplastin times; and fibrinogen concentration.

RESULTS

There were no significant differences detected between results for LR cFFP, compared with those for non-LR cFFP.

CONCLUSIONS AND CLINICAL RELEVANCE

Although there was variation among residual activities of coagulation factors in LR and non-LR cFFP, the variations and differences were considered unlikely to impact the efficacy of LR cFFP transfused for coagulation factor replacement in dogs. However, owing to the small sample size and high variability of results in the present study, additional research with a larger sample size is required for definitive conclusions on the effects of leukoreduction on coagulation factors in cFFP and to develop treatment guidelines for LR cFFP use in dogs with congenital and acquired coagulopathies.

Abstract

OBJECTIVE

To evaluate coagulation factors in units of leukoreduced (LR) and nonleukoreduced (non-LR) canine fresh-frozen plasma (cFFP).

ANIMALS

8 healthy research dogs.

PROCEDURES

In a crossover study, dogs were randomly assigned to 1 of 2 groups from which blood was collected and either did or did not undergo leukoreduction. After a recovery period of ≥ 28 days, the dogs were switched between protocols. After each collection, blood samples were centrifuged, and cFFP was stored frozen for later comparative analysis of coagulation factors, antithrombin, and protein C activities (reported as comparative percentages of the corresponding activities determined in a canine pooled plasma standard); prothrombin and activated partial thromboplastin times; and fibrinogen concentration.

RESULTS

There were no significant differences detected between results for LR cFFP, compared with those for non-LR cFFP.

CONCLUSIONS AND CLINICAL RELEVANCE

Although there was variation among residual activities of coagulation factors in LR and non-LR cFFP, the variations and differences were considered unlikely to impact the efficacy of LR cFFP transfused for coagulation factor replacement in dogs. However, owing to the small sample size and high variability of results in the present study, additional research with a larger sample size is required for definitive conclusions on the effects of leukoreduction on coagulation factors in cFFP and to develop treatment guidelines for LR cFFP use in dogs with congenital and acquired coagulopathies.

Supplementary Materials

    • Supplementary Figure S1 (PDF 155 kb)
    • Supplementary Figure S2 (PDF 135 kb)
    • Supplementary Figure S3 (PDF 134 kb)

Contributor Notes

Dr. Foote's present address is Western Carolina Regional Animal Hospital and Veterinary Emergency Hospital, 205 N Highland Lake Rd, Flat Rock, NC 28731.

Address correspondence to Dr. Thomason (thomason@cvm.msstate.edu).