Editorial Type:
Analytic Techniques

Effect of site of sample collection and prandial state on blood glucose concentrations measured with a portable blood glucose meter in healthy dogs

Jose L. Guevara 1Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Tennessee, Knoxville, TN 37996.

Search for other papers by Jose L. Guevara in
Current site
Google Scholar
PubMed Close
 VMD
,
Karen M. Tobias 1Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Tennessee, Knoxville, TN 37996.

Search for other papers by Karen M. Tobias in
Current site
Google Scholar
PubMed Close
 DVM, MS
,
Jennifer E. Stokes 1Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Tennessee, Knoxville, TN 37996.

Search for other papers by Jennifer E. Stokes in
Current site
Google Scholar
PubMed Close
 DVM
,
Xiaojuan Zhu 2Office of Information Technology, College of Veterinary Medicine, University of Tennessee, Knoxville, TN 37996.

Search for other papers by Xiaojuan Zhu in
Current site
Google Scholar
PubMed Close
 PhD
, and
Rebecca A. Smith 1Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Tennessee, Knoxville, TN 37996.

Search for other papers by Rebecca A. Smith in
Current site
Google Scholar
PubMed Close
 BS
DOI:
https://doi.org/10.2460/ajvr.80.11.995
Volume/Issue:
Volume 80: Issue 11
Online Publication Date:
01 Nov 2019
Restricted access
Sign In Reprints and Permissions Purchase Article

Abstract

OBJECTIVE

To compare glucose concentrations in peripheral venous and capillary blood samples collected from dogs before and after consumption of a meal and measured with a veterinary-specific portable blood glucose meter (PBGM).

ANIMALS

12 dogs (96 blood samples).

PROCEDURES

A veterinary-specific PBGM was used to measure blood glucose concentrations. Glucose concentrations in capillary blood samples obtained from the carpal pad, medial aspect of a pinna, and oral mucosa were compared with glucose concentrations in blood samples obtained from a lateral saphenous vein. Samples were collected after food was withheld for 12 hours and again 2 hours after consumption of a meal.

RESULTS

Location of capillary blood collection had a significant effect on glucose concentrations measured with the PBGM. Glucose concentration in capillary blood collected from the medial aspect of the pinna did not differ significantly from the glucose concentration in peripheral venous blood samples, whereas glucose concentrations in blood samples collected from the carpal pad and oral mucosa differed significantly from the glucose concentration in peripheral venous blood samples. There was no significant difference between preprandial and postprandial blood glucose concentrations.

CONCLUSIONS AND CLINICAL RELEVANCE

Glucose concentrations in capillary blood collected from the medial aspect of the pinna of dogs better reflected glucose concentrations in venous blood than concentrations measured in capillary blood collected from the carpal pad or oral mucosa.

Abstract

OBJECTIVE

To compare glucose concentrations in peripheral venous and capillary blood samples collected from dogs before and after consumption of a meal and measured with a veterinary-specific portable blood glucose meter (PBGM).

ANIMALS

12 dogs (96 blood samples).

PROCEDURES

A veterinary-specific PBGM was used to measure blood glucose concentrations. Glucose concentrations in capillary blood samples obtained from the carpal pad, medial aspect of a pinna, and oral mucosa were compared with glucose concentrations in blood samples obtained from a lateral saphenous vein. Samples were collected after food was withheld for 12 hours and again 2 hours after consumption of a meal.

RESULTS

Location of capillary blood collection had a significant effect on glucose concentrations measured with the PBGM. Glucose concentration in capillary blood collected from the medial aspect of the pinna did not differ significantly from the glucose concentration in peripheral venous blood samples, whereas glucose concentrations in blood samples collected from the carpal pad and oral mucosa differed significantly from the glucose concentration in peripheral venous blood samples. There was no significant difference between preprandial and postprandial blood glucose concentrations.

CONCLUSIONS AND CLINICAL RELEVANCE

Glucose concentrations in capillary blood collected from the medial aspect of the pinna of dogs better reflected glucose concentrations in venous blood than concentrations measured in capillary blood collected from the carpal pad or oral mucosa.

Contributor Notes

Address correspondence to Dr. Guevara (jlguevara316@gmail.com).
Cover American Journal of Veterinary Research
American Journal of Veterinary Research
Publication Date:
01 Nov 2019

  • 1. Casella M, Wess G, Reusch CE. Measurement of capillary blood glucose concentrations by pet owners: a new tool in the management of diabetes mellitus. J Am Anim Hosp Assoc 2002;38:239–245.

    • Crossref
    • PubMed
    • Search Google Scholar
    • Export Citation

  • 2. Wess G, Reusch C. Capillary blood sampling from the ear of dogs and cats and use of portable meters to measure glucose concentration. J Small Anim Pract 2000;41:60–66.

    • Crossref
    • PubMed
    • Search Google Scholar
    • Export Citation

  • 3. Wess G, Reusch C. Evaluation of five portable blood glucose meters for use in dogs. J Am Vet Med Assoc 2000;216:203–209.

  • 4. Wess G, Reusch C. Assessment of five portable blood glucose meters for use in cats. Am J Vet Res 2000;61:1587–1592.

  • 5. Kang MH, Kim DH, Jeong IS, et al. Evaluation of four portable blood glucose meters in diabetic and non-diabetic dogs and cats. Vet Q 2016;36:2–9.

  • 6. Mori A, Oda H, Onozawa E, et al. Evaluation of newly developed veterinary portable blood glucose meter with hematocrit correction in dogs and cats. J Vet Med Sci 2017;79:1690–1693.

    • Crossref
    • PubMed
    • Search Google Scholar
    • Export Citation

  • 7. Cohn LA, McCaw DL, Tate DJ, et al. Assessment of five portable blood glucose meters, a point-of-care analyzer, and color test strips for measuring blood glucose concentration in dogs. J Am Vet Med Assoc 2000;216:198–202.

    • Crossref
    • PubMed
    • Search Google Scholar
    • Export Citation

  • 8. Cohen TA, Nelson RW, Kass PH, et al. Evaluation of six portable blood glucose meters for measuring blood glucose concentration in dogs. J Am Vet Med Assoc 2009;235:276–280.

    • Crossref
    • PubMed
    • Search Google Scholar
    • Export Citation

  • 9. Zoetis Inc. AlphaTRAK 2 blood glucose test strips, product information, 2018. Kalamazoo, Mich: Zoetis Inc. Available at: www.zoetisus.com/contact/pages/product_information/msds_pi/pi/alphatrak_2.pdf. Accessed Aug 9, 2018.

    • Search Google Scholar
    • Export Citation

  • 10. Zoetis Inc. AlphaTRAK blood glucose monitoring system, quick user's guide, 2018. Kalamazoo, Mich: Zoetis Inc. Available at: www.zoetisus.com/products/dogs/alphatrakmeter/pdf/alphatrak-2-quick-user_s-guide.pdf. Accessed Aug 9, 2018.

    • Search Google Scholar
    • Export Citation

  • 11. Borin S, Crivelenti LZ, Hoeppner Rondelli MC, et al. Capillary blood glucose and venous blood glucose measured with portable digital glucometer in diabetic dogs. Braz J Vet Pathol 2012;5:42–46.

    • Search Google Scholar
    • Export Citation

  • 12. Togashi Y, Shirakawa J, Okuyama T, et al. Evaluation of the appropriateness of using glucometers for measuring the blood glucose levels in mice. Sci Rep 2016;6:25465.

  • 13. Juneja D, Pandey R, Singh O. Comparison between arterial and capillary blood glucose monitoring in patients with shock. Eur J Intern Med 2011;22:241–244.

    • Crossref
    • PubMed
    • Search Google Scholar
    • Export Citation

  • 14. Bland JM, Altman G. Statistical methods for assessing agreement between two methods of clinical measurement. Lancet 1986;1:307–310.

  • 15. Parkes JL, Slatin SL, Pardo S, et al. A new consensus error grid to evaluate the clinical significance of inaccuracies in the measurement of blood glucose. Diabetes Care 2000;23:1143–1148.

    • Crossref
    • PubMed
    • Search Google Scholar
    • Export Citation

  • 16. Hall JE. Guyton and Hall textbook of medical physiology. 11th ed. Philadelphia: Elsevier, 2016;971–972.

Advertisement