• 1. Rechner AR. Platelet function testing in clinical diagnostics. Hamostaseologie 2011; 31:7987.

  • 2. Pakala R, Waksman R. Currently available methods for platelet function analysis: advantages and disadvantages. Cardiovasc Revasc Med 2011; 12:312322.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 3. Wiinberg B, Jessen LR, Tarnow I, et al. Diagnosis and treatment of platelet hyperactivity in relation to thrombosis in dogs and cats. J Vet Emerg Crit Care (San Antonio) 2012; 22:4258.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 4. Würtz M, Hvas AM, Christensen KH, et al. Rapid evaluation of platelet function using the Multiplate analyzer. Platelets 2014; 25:628633.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 5. Kalbantner K, Baumgarten A, Mischke R. Measurement of platelet function in dogs using a novel impedance aggregometer. Vet J 2010; 185:144151.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 6. Marschner CB, Kristensen AT, Spodsberg EH, et al. Evaluation of platelet aggregometry in dogs using the Multiplate platelet analyzer: impact of anticoagulant choice and assay duration. J Vet Emerg Crit Care (San Antonio) 2012; 22:107115.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 7. Li RH, Chan DL. Evaluation of platelet function using multiple electrode platelet aggregometry in septic peritonitis. J Vet Emerg Crit Care (San Antonio) 2016; 26:630638.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 8. Adamzik M, Görlinger K, Peters J, et al. Whole blood impedance aggregometry as a biomarker for the diagnosis and prognosis of severe sepsis. Crit Care 2012; 16:R204.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 9. Stissing T, Dridi NP, Ostrowski SR, et al. The influence of low platelet count on whole blood aggregometry assessed by Multiplate. Clin Appl Thromb Hemost 2011; 17:E211E217.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 10. Femia EA, Scavone M, Lecchi A, et al. Effect of platelet count on platelet aggregations measured with impedance aggregometry (Multiplate analyzer) and with light transmission aggregometry. J Thromb Haemost 2013; 11:21932196.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 11. Rubak P, Villadsen K, Hvas AM. Reference intervals for platelet aggregation assessed by multiple electrode platelet aggregometry. Thromb Res 2012; 130:420423.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 12. Würtz M, Hvas AM, Kristensen SD, et al. Platelet aggregation is dependent on platelet count in patients with coronary artery disease. Thromb Res 2012; 129:5661.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 13. Hanke AA, Roberg K, Monaca E, et al. Impact of platelet count on results obtained from multiple electrode platelet aggregometry (Multiplate). Eur J Med Res 2010; 15:214219.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 14. Boudreaux MK, Lipscomb DL. Clinical, biochemical, and molecular aspects of Glanzmann's thrombasthenia in humans and dogs. Vet Pathol 2001; 38:249260.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 15. Locke K. Severe haemorrhage in Dobermann dogs with von Willebrand's disease and its control during surgery. Aust Vet J 1994; 71:263.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 16. Singh MK, Lamb WA. Idiopathic thrombocytopenia in Cavalier King Charles Spaniels. Aust Vet J 2005; 83:700703.

  • 17. Bates D, Maechler M, Bolker B, et al. Fitting linear mixed-effects models using lme4. J Stat Softw 2015; 67:148.

  • 18. Otahbachi M, Simoni J, Simoni G, et al. Gender differences in platelet aggregation in healthy individuals. J Thromb Thrombolysis 2010; 30:184191.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 19. Nemeth N, Kiss F, Furka I, et al. Gender differences of blood rheological parameters in laboratory animals. Clin Hemorheol Microcirc 2010; 45:263272.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 20. Melamed N, Yogev Y, Bouganim T, et al. The effect of menstrual cycle on platelet aggregation in reproductive-age women. Platelets 2010; 21:343347.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 21. Stafford NP, Pink AE, White AE, et al. Mechanisms involved in adenosine triphosphate–induced platelet aggregation in whole blood. Arterioscler Thromb Vasc Biol 2003; 23:19281933.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 22. Renesto P, Chignard M. Neutrophil-mediated platelet activation: a key role for serine proteases. Gen Pharmacol 1995; 26:905910.

  • 23. Oleksowicz L, Paciucci PA, Zuckerman D, et al. Alterations of platelet function induced by interleukin-2. J Immunother 1991; 10:363370.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 24. Becatti M, Fiorillo C, Gori AM, et al. Platelet and leukocyte ROS production and lipoperoxidation are associated with high platelet reactivity in Non-ST elevation myocardial infarction (NSTEMI) patients on dual antiplatelet treatment. Atherosclerosis 2013; 231:392400.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 25. Valles J, Santos MT, Marcus AJ, et al. Downregulation of human platelet reactivity by neutrophils. J Clin Invest 1993; 92:13571365.

  • 26. Mackie IJ, Jones R, Machin SJ. Platelet impedance aggregation in whole blood and its inhibition by antiplatelet drugs. J Clin Pathol 1984; 37:874878.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 27. Sweeney JD, Labuzetta JW, Fitzpatrick JE. The effect of platelet count on the aggregation response and adenosine triphosphate release in an impedance lumi-aggregometer. Am J Clin Pathol 1988; 89:655659.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 28. Tvedten H, Lilliehöök I, Hillström A, et al. Plateletcrit is superior to platelet count for assessing platelet status in Cavalier King Charles Spaniels. Vet Clin Pathol 2008; 37:266271.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 29. Müller MR, Salat A, Pulaki S, et al. Influence of hematocrit and platelet count on impedance and reactivity of whole blood for electrical aggregometry. J Pharmacol Toxicol Methods 1995; 34:1722.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 30. Dyszkiewicz-Korpanty AM, Frenkel EP, Sarode P. Approach to the assessment of platelet function: comparison between optical-based platelet-rich plasma and impedance-based whole blood platelet aggregation methods. Clin Appl Thromb Hemost 2005; 11:2535.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 31. Bauer NB, Er E, Moritz A. Influence of blood collection technique on platelet function and coagulation variables in dogs. Am J Vet Res 2011; 72:6472.

    • Crossref
    • Search Google Scholar
    • Export Citation

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In vitro effect of blood cell counts on multiple-electrode impedance aggregometry in dogs

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  • 1 Department of Clinical Sciences, College of Veterinary Medicine, Auburn University, Auburn, AL 36849.
  • | 2 Department of Clinical Sciences, College of Veterinary Medicine, Auburn University, Auburn, AL 36849.
  • | 3 Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL 36849.
  • | 4 Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL 36849.

Abstract

OBJECTIVE To assess the effect of decreased platelet and WBC counts on platelet aggregation as measured by a multiple-electrode impedance aggregometer in dogs.

ANIMALS 24 healthy dogs.

PROCEDURES From each dog, 9 mL of blood was collected into a 10-mL syringe that contained 1 mL of 4% sodium citrate solution to yield a 10-mL sample with a 1:9 citrate-to-blood ratio. Each sample was then divided into unmanipulated and manipulated aliquots with progressively depleted buffy-coat fractions such that 2 to 3 blood samples were evaluated per dog. The Hct for manipulated aliquots was adjusted with autologous plasma so that it was within 2% of the Hct for the unmanipulated aliquot for each dog. All samples were analyzed in duplicate with a multiple-electrode impedance aggregometer following the addition of ADP as a platelet agonist. The respective effects of platelet count, plateletcrit, Hct, and WBC count on platelet aggregation area under the curve (AUC), aggregation, and velocity were analyzed with linear mixed models.

RESULTS WBC count was positively associated with platelet AUC, aggregation, and velocity; blood samples with leukopenia had a lower AUC, aggregation, and velocity than samples with WBC counts within the reference range. Platelet count, plateletcrit, and Hct did not have an independent effect on AUC, aggregation, or velocity.

CONCLUSIONS AND CLINICAL RELEVANCE Results indicated that WBC count was positively associated with platelet aggregation when ADP was used to activate canine blood samples for impedance aggregometry. That finding may be clinically relevant and needs to be confirmed by in vivo studies.

Abstract

OBJECTIVE To assess the effect of decreased platelet and WBC counts on platelet aggregation as measured by a multiple-electrode impedance aggregometer in dogs.

ANIMALS 24 healthy dogs.

PROCEDURES From each dog, 9 mL of blood was collected into a 10-mL syringe that contained 1 mL of 4% sodium citrate solution to yield a 10-mL sample with a 1:9 citrate-to-blood ratio. Each sample was then divided into unmanipulated and manipulated aliquots with progressively depleted buffy-coat fractions such that 2 to 3 blood samples were evaluated per dog. The Hct for manipulated aliquots was adjusted with autologous plasma so that it was within 2% of the Hct for the unmanipulated aliquot for each dog. All samples were analyzed in duplicate with a multiple-electrode impedance aggregometer following the addition of ADP as a platelet agonist. The respective effects of platelet count, plateletcrit, Hct, and WBC count on platelet aggregation area under the curve (AUC), aggregation, and velocity were analyzed with linear mixed models.

RESULTS WBC count was positively associated with platelet AUC, aggregation, and velocity; blood samples with leukopenia had a lower AUC, aggregation, and velocity than samples with WBC counts within the reference range. Platelet count, plateletcrit, and Hct did not have an independent effect on AUC, aggregation, or velocity.

CONCLUSIONS AND CLINICAL RELEVANCE Results indicated that WBC count was positively associated with platelet aggregation when ADP was used to activate canine blood samples for impedance aggregometry. That finding may be clinically relevant and needs to be confirmed by in vivo studies.

Contributor Notes

Dr. Nash's present address is VETMED, 20610 N Cave Creek Rd, Phoenix, AZ 85024.

Address correspondence to Dr. Nash (kjnash1@outlook.com).