Editorial Type:
Ophthalmology

Use of indocyanine green and sodium fluorescein for anterior segment angiography in ophthalmologically normal cats

Chris G. Pirie Department of Clinical Sciences, Cummings School of Veterinary Medicine, Tufts University, North Grafton, MA 01536.

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Anthony Alario Department of Clinical Sciences, Cummings School of Veterinary Medicine, Tufts University, North Grafton, MA 01536.

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DOI:
https://doi.org/10.2460/ajvr.76.10.897
Volume/Issue:
Volume 76: Issue 10
Online Publication Date:
01 Oct 2015
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Abstract

OBJECTIVE To assess and compare results of anterior segment angiography of ophthalmologically normal cats following IV injection with indocyanine green and sodium fluorescein dyes.

ANIMALS 10 client-owned cats.

PROCEDURES Anterior segment angiography was performed in anesthetized cats following administration of 0.25% indocyanine green (1.0 mg/kg, IV) or 10% sodium fluorescein (20 mg/kg, IV) solution. All cats received both treatments. Imaging (1 eye/cat) was performed with a full-spectrum digital single-lens reflex camera equipped with an adaptor (1 image/s for 30 seconds) immediately following IV dye injection and 1, 2, 3, 4, and 5 minutes after injection. Onset and duration of arterial, capillary, and venous phases of iris vasculature were identified and compared statistically between treatments. Degree of iridal pigmentation, leakage of dye from iris vasculature, and image quality were subjectively assessed.

RESULTS No differences were found in onset or duration of vascular phases between treatments. Visibility of the iris vasculature was not impaired by poor or moderate iridal pigmentation with either method. Indocyanine green provided subjectively better vascular detail and image contrast than sodium fluorescein. No vascular dye leakage was observed following indocyanine green administration. Leakage of dye from blood vessels in the stroma (in 10 cats) and presence of dye in the anterior chamber (in 5 cats) were detected after sodium fluorescein administration.

CONCLUSIONS AND CLINICAL RELEVANCE Images obtained with either fluorescent dye were considered to be of diagnostic quality. Lack of leakage following indocyanine green administration suggested this treatment may have better diagnostic utility for anterior segment angiography. The photographic equipment used provided a cost-effective alternative to existing imaging systems.

Abstract

OBJECTIVE To assess and compare results of anterior segment angiography of ophthalmologically normal cats following IV injection with indocyanine green and sodium fluorescein dyes.

ANIMALS 10 client-owned cats.

PROCEDURES Anterior segment angiography was performed in anesthetized cats following administration of 0.25% indocyanine green (1.0 mg/kg, IV) or 10% sodium fluorescein (20 mg/kg, IV) solution. All cats received both treatments. Imaging (1 eye/cat) was performed with a full-spectrum digital single-lens reflex camera equipped with an adaptor (1 image/s for 30 seconds) immediately following IV dye injection and 1, 2, 3, 4, and 5 minutes after injection. Onset and duration of arterial, capillary, and venous phases of iris vasculature were identified and compared statistically between treatments. Degree of iridal pigmentation, leakage of dye from iris vasculature, and image quality were subjectively assessed.

RESULTS No differences were found in onset or duration of vascular phases between treatments. Visibility of the iris vasculature was not impaired by poor or moderate iridal pigmentation with either method. Indocyanine green provided subjectively better vascular detail and image contrast than sodium fluorescein. No vascular dye leakage was observed following indocyanine green administration. Leakage of dye from blood vessels in the stroma (in 10 cats) and presence of dye in the anterior chamber (in 5 cats) were detected after sodium fluorescein administration.

CONCLUSIONS AND CLINICAL RELEVANCE Images obtained with either fluorescent dye were considered to be of diagnostic quality. Lack of leakage following indocyanine green administration suggested this treatment may have better diagnostic utility for anterior segment angiography. The photographic equipment used provided a cost-effective alternative to existing imaging systems.

Contributor Notes

Dr. Alario's present address is Capital Area Veterinary Emergency Service, 1 Intervale Rd, Concord, NH 03301.

Address correspondence to Dr. Pirie (chris.pirie@tufts.edu).
Cover American Journal of Veterinary Research
American Journal of Veterinary Research
Publication Date:
01 Oct 2015

  • 1. Brancato R, Bandello F, Lattanzio R. Iris fluorescein angiography in clinical practice. Surv Ophthalmol 1997; 42: 41–70.

  • 2. Chan TK, Rosenbaum AL, Rao R, et al. Indocyanine green angiography of the anterior segment in patients undergoing strabismus surgery. Br J Ophthalmol 2001; 85: 214–218.

    • Search Google Scholar
    • Export Citation

  • 3. Pitet G, Amalric P, Hygounenc O. Etude anlytique de la fluorescence des solutions de fluoresceinate de sodium In: Amalric P, ed. Fluorescein angiography: proceedings of the international symposium on fluorescein angiography, Albi, France, 1969. Basel, Switzerland: S. Karger, 1971;8–11.

    • Search Google Scholar
    • Export Citation

  • 4. Novotny HR, Alvis DL. A method of photographing fluorescence in circulating blood in the human retina. Circulation 1961; 24: 82–86.

    • Search Google Scholar
    • Export Citation

  • 5. Baggesen LH. Fluorescence angiography of the iris in diabetics and non-diabetics. Acta Ophthalmol (Copenh) 1969; 47: 449–460.

  • 6. Mapstone R. Fluorescein iridography. Br J Ophthalmol 1971; 55: 400–406.

  • 7. Vannas A. Fluorescein angiography of the vessels of the iris in pseudoexfoliation of the lens capsule, capsular glaucoma and some other forms of glaucoma. Acta Ophthalmol Suppl 1969; 105: 1–75.

    • Search Google Scholar
    • Export Citation

  • 8. Bandello F, Brancato R, Lattanzio R, et al. Biomicroscopy and fluorescein angiography of pigmented iris tumors. A retrospective study on 44 cases. Int Ophthalmol 1994; 18: 61–70.

    • Search Google Scholar
    • Export Citation

  • 9. Maruyama Y, Kishi S, Kamei Y, et al. Infrared angiography of the anterior ocular segment. Surv Ophthalmol 1995; 39 (suppl 1): S40–S48.

    • Search Google Scholar
    • Export Citation

  • 10. Landsman ML, Kwant G, Mook GA, et al. Light-absorbing properties, stability, and spectral stabilization of indocyanine green. J Appl Physiol 1976; 40: 575–583.

    • Search Google Scholar
    • Export Citation

  • 11. Mordon S, Devoisselle JM, Soulie-Begu S, et al. Indocyanine green: physicochemical factors affecting its fluorescence in vivo. Microvasc Res 1998; 55: 146–152.

    • Search Google Scholar
    • Export Citation

  • 12. Desmettre T, Devoisselle JM, Mordon S. Fluorescence properties and metabolic features of indocyanine green (ICG) as related to angiography. Surv Ophthalmol 2000; 45: 15–27.

    • Search Google Scholar
    • Export Citation

  • 13. Maarek JM, Holschneider DP, Harimoto J. Fluorescence of indocyanine green in blood: intensity dependence on concentration and stabilization with sodium polyaspartate. J Photochem Photobiol B 2001; 65: 157–164.

    • Search Google Scholar
    • Export Citation

  • 14. Guex-Crosier Y, Durig J. Anterior segment indocyanine green angiography in anterior scleritis and episcleritis. Ophthalmology 2003; 110: 1756–1763.

    • Search Google Scholar
    • Export Citation

  • 15. Ishibashi S, Tawara A, Sohma R, et al. Angiographic changes in iris and iridocorneal angle neovascularization after intravitreal bevacizumab injection. Arch Ophthalmol 2010; 128: 1539–1545.

    • Search Google Scholar
    • Export Citation

  • 16. Baglio V, Gharbiya M, Balacco-Gabrieli C, et al. Choroidopathy in patients with systemic lupus erythematosus with or without nephropathy. J Nephrol 2011; 24: 522–529.

    • Search Google Scholar
    • Export Citation

  • 17. Gharbiya M, Pecci G, Baglio V, et al. Indocyanine green angiographic findings for patients with systemic lupus erythematosus nephropathy. Retina 2006; 26: 159–164.

    • Search Google Scholar
    • Export Citation

  • 18. Zuo CG, Wen F, Huang SZ, et al. Angiographic leakage of polypoidal choroidal vasculopathy on indocyanine angiography. Chin Med J (Engl) 2010; 123: 1548–1552.

    • Search Google Scholar
    • Export Citation

  • 19. Kobayashi T, Kubo E, Takahashi Y, et al. Retinal vessel changes in galactose-fed dogs. Arch Ophthalmol 1998; 116: 785–789.

  • 20. Ld S, Simoens P, Lauwers H. Fluorescein angiography of the canine retina. Vet Comp Ophthalmol 1996; 6: 111–119.

  • 21. Narfstrom K, Vaegan, Katz M, et al. Assessment of structure and function over a 3-year period after gene transfer in RPE65–/–dogs. Documenta Ophthalmol 2005; 111: 39–48.

    • Search Google Scholar
    • Export Citation

  • 22. Pizzirani S, Davidson MG, Gilger BC. Transpupillary diode laser retinopexy in dogs: ophthalmoscopic, fluorescein angiographic and histopathologic study. Vet Ophthalmol 2003; 6: 227–235.

    • Search Google Scholar
    • Export Citation

  • 23. Hill DW, Young S. Arterial fluorescence angiography of the fundus oculi of the cat: appearances and measurements. Exp Eye Res 1973; 16: 457–465.

    • Search Google Scholar
    • Export Citation

  • 24. Hill DW, Houseman J. Retinal blood flow to tapetal and pigmented fundus in the cat. Exp Eye Res 1980; 30: 245–252.

  • 25. Linsenmeier RA, Braun RD, McRipley MA, et al. Retinal hypoxia in long-term diabetic cats. Invest Ophthalmol Vis Sci 1998; 39: 1647–1657.

    • Search Google Scholar
    • Export Citation

  • 26. Lee CJ, Smith JH, Kang-Mieler JJ, et al. Decreased circulation in the feline choriocapillaris underlying retinal photocoagulation lesions. Invest Ophthalmol Vis Sci 2011; 52: 3398–3403.

    • Search Google Scholar
    • Export Citation

  • 27. Alario AF, Pirie CG, Pizzirani S. Anterior segment fluorescein angiography of the normal canine eye using a DSLR camera adapter. Vet Ophthalmol 2013; 16: 10–19.

    • Search Google Scholar
    • Export Citation

  • 28. Alario AF, Pirie CG, Pizzirani S. Anterior segment fluorescein angiography of the normal feline eye using a dSLR camera adaptor. Vet Ophthalmol 2013; 16: 204–213.

    • Search Google Scholar
    • Export Citation

  • 29. Grahn BH, Sandmeyer LL, Breaux C. Retinopathy of coton de tulear dogs: clinical manifestations, electroretinographic, ultrasonographic, fluorescein and indocyanine green angiographic, and optical coherence tomographic findings. Vet Ophthalmol 2008; 11: 242–249.

    • Search Google Scholar
    • Export Citation

  • 30. Hochheimer BF. Angiography of the retina with indocyanine green. Arch Ophthalmol 1971; 86: 564–565.

  • 31. Hill DW, Young S. Infrared angiography of the cat fundus oculi. Arch Ophthalmol 1975; 93: 131–133.

  • 32. Hill DW, Houseman J. Retinal blood flow in the cat following periods of light and darkness. Exp Eye Res 1985; 41: 219–225.

  • 33. Seiler MJ, Aramant RB, Seeliger MW, et al. Functional and structural assessment of retinal sheet allograft transplantation in feline hereditary retinal degeneration. Vet Ophthalmol 2009; 12: 158–169.

    • Search Google Scholar
    • Export Citation

  • 34. Panzardi G, Donati MC, Longobardi G, et al. Choroidal angiography with indocyanine green dye: absorption and fluorescence techniques. Eur J Ophthalmol 1992; 2: 83–85.

    • Search Google Scholar
    • Export Citation

  • 35. Pirie CG, Alario A. Anterior segment angiography of the normal canine eye: a comparison between indocyanine green and sodium fluorescein. Vet J 2014; 199: 360–364.

    • Search Google Scholar
    • Export Citation

  • 36. Pirie CG, Pizzirani S. Anterior and posterior segment photography. An alternative approach using a dSLR camera adaptor. Vet Ophthalmol 2012; 15: 280–287.

    • Search Google Scholar
    • Export Citation

  • 37. Kottow M. Anterior segment fluorescein angiography. Baltimore: Williams and Wilkins, 1978.

  • 38. Saari M. Ultrastructure of the microvessels of the iris in mammals with special reference to their permeability. Albrecht Von Graefes Arch Klin Exp Ophthalmol 1975; 194: 87–93.

    • Search Google Scholar
    • Export Citation

  • 39. Szalay J, Nunziata B, Henkind P. Permeability of iridial blood vessels. Exp Eye Res 1975; 21: 531–543.

  • 40. Bellhorn RW. Permeability of blood-ocular barriers of neonatal and adult cat to sodium fluorescein. Invest Ophthalmol Vis Sci 1980; 19: 870–877.

    • Search Google Scholar
    • Export Citation

  • 41. May CA, Lutjen-Drecoll E, Narfstrom K. Morphological changes in the anterior segment of the Abyssinian cat eye with hereditary rod-cone degeneration. Curr Eye Res 2005; 30: 855–862.

    • Search Google Scholar
    • Export Citation

  • 42. Nilsson SF, Maepea O, Alm A, et al. Ocular blood flow and retinal metabolism in Abyssinian cats with hereditary retinal degeneration. Invest Ophthalmol Vis Sci 2001; 42: 1038–1044.

    • Search Google Scholar
    • Export Citation

  • 43. Bischoff PM, Niederberger HJ, Torok B, et al. Simultaneous indocyanine green and fluorescein angiography. Retina 1995; 15: 91–99.

    • Search Google Scholar
    • Export Citation

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