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Simplified procedure for the estimation of glomerular filtration rate following intravenous administration of iodixanol in cats

Rieko Katayama DVM, PhD1, Jun Saito DVM2, Masaaki Katayama DVM, PhD3, Norio Yamagishi DVM, PhD4, Tetsuro Yamashita PhD5, Masaki Kato DVM6, and Kazuhisa Furuhama DVM, PhD7
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  • 1 Department of Veterinary Basic Medicine, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan.
  • | 2 Department of Veterinary Basic Medicine, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan.
  • | 3 Veterinary Clinical Medicine, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan.
  • | 4 Veterinary Clinical Medicine, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan.
  • | 5 Food Science and Biochemistry, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan.
  • | 6 Department of Veterinary Basic Medicine, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan.
  • | 7 Department of Veterinary Basic Medicine, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan.

Abstract

Objective—To compare the use of a single-sample method involving IV administration of iodixanol with a multisample method involving inulin for the estimation of glomerular filtration rate (GFR) in cats.

Animals—24 cats, including 15 healthy cats and 9 cats with naturally occurring renal diseases.

Procedures—Each cat was coadministered iodixanol (a nonionic contrast medium; dose providing 40 mg of I/kg) and inulin (50 mg/kg), IV, and blood samples were collected 60, 90, and 120 minutes later. Serum iodixanol and inulin concentrations were determined by means of high-performance liquid chromatography and colorimetry, respectively. Serum urea nitrogen and creatinine concentrations were also measured.

Results—Analysis of the data from healthy cats and cats with naturally occurring renal diseases revealed an excellent correlation between GFR values estimated by the multisample and single-sample methods with iodixanol. Likewise, GFR values estimated from the single-sample method with iodixanol were closely correlated with those calculated from the multisample method with inulin.

Conclusions and Clinical Relevance—For estimation of GFR in cats, use of a single-sample method with iodixanol, instead of a multisample procedure, may be an expedient tool in both clinical and research settings because of its benefits to patient well-being as a result of reduced stress associated with blood sample collection.

Contributor Notes

The authors thank Drs. Kyoko Imai and Yuuki Michigoshi for technical assistance.

Address correspondence to Dr. Furuhama (furuhama@iwate-u.ac.jp).