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1 Lindholm A, Saltin B. The physiological and biochemical response of Standardbred horses to exercise of varying speed and duration. Acta Vet Scand 1974; 15:310–324.
-
2 Valberg S, Gustavsson BE, Lindholm A, et al. Blood chemistry and skeletal muscle metabolic responses during and after different speeds and durations of trotting. Equine Vet J 1989; 2:91–95.
-
3 Schuback K, Essén-Gustavsson B. Muscle anaerobic response to a maximal treadmill exercise test in Standardbred trotters. Equine Vet J 1998; 30:504–510.
-
4 Essén-Gustavsson B, Karlström K, Lindholm A. Fibre types, enzyme activities and substrate utilization in skeletal muscle of horses competing in endurance rides. Equine Vet J 1984; 16:197–202.
-
5 Essén-Gustavsson B, Jensen-Waern M. Effect of an endurance race on muscle amino acids, pro- and macroglycogen and triglycerids. Equine Vet J Suppl2002;(34):209–213.
-
6 Bröjer J, Holm S, Jonasson R, et al. Synthesis of proglycogen and macroglycogen in skeletal muscle of Standardbred trotters after intermittent exercise. Equine Vet J Suppl2006;(36):335–339.
-
7 Hyyppä S, Räsänen L, Pösö R. Resynthesis of glycogen in skeletal muscle from Standardbred trotters after repeated bouts of exercise. Am J Vet Res 1997; 58:162–166.
-
8 Lacombe VA, Hinchcliff KW, Kohn CW, et al. Effects of feeding meals with various soluble-carbohydrate content on muscle glycogen synthesis after exercise in horses. Am J Vet Res 2004; 65:916–923.
-
9 Jose-Cunilleras E, Hinchcliff KW, Lacombe VA, et al. Ingestion of starch-rich meals after exercise increases glucose kinetics but fails to enhance muscle glycogen replenishment in horses. Vet J 2006; 171:468–477.
-
10 Bergström J, Hermansen L, Hultman E, et al. Diet, muscle glycogen and physical performance. Acta Physiol Scand 1967; 71:140–150.
-
11 Jentjens R, Jeukendrup A. Determinants of post-exercise glycogen storage during short-term recovery. Sports Med 2003; 33:117–144.
-
12 Ivy JL, Lee MC, Brozonick JT, et al. Muscle glycogen storage after different amounts of carbohydrate ingestion. J Appl Physiol 1988; 65:2018–2023.
-
13 Davie AJ, Evans DL, Hodgson DR, et al. Effects of intravenous dextrose infusion on muscle glycogen storage after intense exercise. Equine Vet J Suppl1995;(18):195–198.
-
14 Geor RJ, Larsen L, Waterfall HL, et al. Route of carbohydrate administration affects early post exercise muscle glycogen storage in horses. Equine Vet J Suppl2006;(34):590–595.
-
15 Ivy JL, Kuo CH. Regulation of GLUT4 protein and glycogen synthase during muscle glycogen synthesis after exercise. Acta Physiol Scand 1998; 162:295–304.
-
16 van Loon LJ, Saris WH, Verhagen H, et al. Plasma insulin responses after ingestion of different amino acid or protein mixtures with carbohydrate. Am J Clin Nutr 2000; 72:96–105.
-
17 van Loon LJ, Saris WH, Kruijshoop M, et al. Maximizing postexercise muscle glycogen synthesis: carbohydrate supplementation and the application of amino acid or protein hydrolysate mixtures. Am J Clin Nutr 2000; 72:106–111.
-
18 Zawadzki KM, Yaspelskis BB III, Ivy JL. Carbohydrate-protein complex increases the rate of muscle glycogen storage after exercise. J Appl Physiol 1992; 75:1854–1859.
-
19 van Loon LJ, Kruijshoop M, Verhagen H, et al. Ingestion of protein hydrolysate and amino acid-carbohydrate mixtures increases postexercise plasma insulin responses in men. J Nutr 2000; 130:2508–2513.
-
20 Jentjens RL, van Loon LJ, Mann CH, et al. Addition of protein and amino acids to carbohydrates does not enhance postexercise muscle glycogen synthesis. J Appl Physiol 2001; 91:839–846.
-
21 Pösö RA, Hyyppä S. Metabolic and hormonal changes after exercise in relation to muscle glycogen concentrations. Equine Vet J Suppl 1999; 30:332–336.
-
22 Lindgren E. Vallfodrets näringsvärde bestämt in vivo och med olika laboratoriemetoder (Forage analysis-methods descriptions for sampling and analysis). Report 45. Uppsala, Sweden: Research Information Centre, Department of Animal Nutrition and Management, Swedish University of Agricultural Sciences, 1979.
-
23 Jansson A. Utfodringsrekommendationer för häst (Feeding recommendations for horses). Uppsala, Sweden: Department of Equine Studies, Swedish University of Agricultural Sciences, 2004.
-
24 Lindholm A, Piehl K. Fibre composition, enzyme activity and concentration of metabolites and electrolytes in muscles of Standardbred trotters. Acta Vet Scand 1974; 15:287–309.
-
25 Pfeifer R, Karol R, Korpi J, et al. Practical application of HPLC to amino acid analysis. Am Lab 1983; 15:77–84.
-
26 Jonasson R, Essén-Gustavsson B, Jensen-Waern M. Blood concentrations of amino acids, glucose and lactate during experimental swine dysentery. Res Vet Sci 2007; 82:323–331.
-
27 Lowry OH, Passoneau JV. A flexible system of enzymatic analysis. New York: Academic Press, 1973;146–218.
-
28 Cartee GD, Young DA, Slepper MD, et al. Prolonged increase in insulin-stimulated glucose transport in muscle after exercise. Am J Physiol 1989; 256:E494–E499.
-
29 Davie AJ, Evans DL, Hodgson DR, et al. The effects of an oral glucose polymer on muscle glycogen resynthesis in Standardbred horses. J Nutr 1994; 124:2740S–2741S.
-
30 Lacombe VA, Hinchcliff KW, Geor RJ, et al. Muscle glycogen depletion and subsequent replenishment affect anaerobic capacity of horses. J Appl Physiol 2001; 91:1782–1790.
-
31 Pratt SE, Geor RJ, Spriet LL, et al. Time course of insulin sensitivity and skeletal muscle glycogen synthase activity after a single bout of exercise in horses. J Appl Physiol 2007; 103:1063–1069.
-
32 Urschel KL, Geor RJ, Waterfall HL, et al. Effects of leucine or whey protein addition to an oral glucose solution on serum insulin, plasma glucose and plasma amino acid responses in horses at rest and following exercise. Equine Vet J 2010; 42(suppl 38): 347–354.
-
33 Lacombe VA, Hinchcliff KW, Devor ST. Effects of exercise and glucose administration on content of insulin-sensitive glucose transporter in equine skeletal muscle. Am J Vet Res 2003; 64:1500–1506.
-
34 Waller AP, Geor RJ, Spriet LL, et al. Oral acetate supplementation after prolonged moderate intensity exercise enhances early muscle glycogen resynthesis in horses. Exp Physiol 2009; 94:888–898.
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Effect of repeated oral administration of glucose and leucine immediately after exercise on plasma insulin concentration and glycogen synthesis in horses
Abstract
Objective—To determine whether repeated oral administration of glucose and leucine during the period immediately after intense exercise would increase the release of insulin and thereby enhance glycogen synthesis in horses.
Animals—12 Standardbred horses.
Procedures—In a crossover study design, after glycogen-depleting exercise, horses received oral boluses of glucose (1 g/kg at 0, 2, and 4 hours) and leucine (0.1 g/kg at 0 and 4 hours) or boluses of water (10 mL/kg at 0, 2, and 4 hours; control treatment). Blood samples for determination of glucose, insulin, and leucine concentrations were collected prior to and during a 6-hour period immediately after exercise. Biopsy specimens of a gluteus muscle were obtained before and immediately after exercise and at 3, 6, and 24 hours after exercise for measurement of glycogen concentration.
Results—When glucose and leucine were administered to the horses, plasma insulin concentration was significantly higher during the 6 hours immediately after exercise than it was when water was administered to the horses. Serum glucose concentration during the 4 hours immediately after exercise was significantly higher when glucose and leucine were administered than the serum glucose concentration when water was administered. Muscle glycogen concentrations did not differ between the 2 treatments during the 24 hours after exercise.
Conclusions and Clinical Relevance—Synthesis of muscle glycogen after intense intermittent exercise was not enhanced by oral boluses of glucose and leucine after exercise despite pronounced increases in plasma insulin and serum glucose concentrations.
Contributor Notes
Supported by the Swedish-Norwegian Foundation for Equine Research.
The authors thank Dr. Ulf Emanuelsson for assistance with statistical analyses.
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