Prevalence of perinuclear antineutrophilic cytoplasmic autoantibodies in serum of healthy Soft Coated Wheaten Terriers in the United Kingdom

Barbara Wieland Department of Veterinary Clinical Sciences, Royal Veterinary College, University of London, North Mymms, Hatfield, Hertfordshire, AL9 7TA, England.

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 Dr med vet, PhD
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Jennifer F. Summers Department of Veterinary Clinical Sciences, Royal Veterinary College, University of London, North Mymms, Hatfield, Hertfordshire, AL9 7TA, England.

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Barbara Häsler Department of Veterinary Clinical Sciences, Royal Veterinary College, University of London, North Mymms, Hatfield, Hertfordshire, AL9 7TA, England.

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Carolina Mancho-Alonso Department of Animal Medicine and Surgery, College of Veterinary Medicine, Complutense University of Madrid, Madrid, Spain.

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Amanda Craig Department of Veterinary Clinical Sciences, Royal Veterinary College, University of London, North Mymms, Hatfield, Hertfordshire, AL9 7TA, England.

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Karin Allenspach Department of Veterinary Clinical Sciences, Royal Veterinary College, University of London, North Mymms, Hatfield, Hertfordshire, AL9 7TA, England.

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DOI:
https://doi.org/10.2460/ajvr.73.3.404
Volume/Issue:
Volume 73: Issue 3
Online Publication Date:
01 Mar 2012
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Abstract

Objective—To estimate the prevalence of perinuclear antineutrophilic cytoplasmic autoantibodies (pANCA) in the serum of healthy Soft Coated Wheaten Terriers (SCWTs) in the United Kingdom and to identify potential risk factors and heritability patterns associated with a positive result for pANCA.

Animals—188 SCWTs (age range, 18 months to 14.3 years).

Procedures—Blood samples were obtained from SCWTs in various locations in England. Serum was tested for pANCA by use of an immunofluorescence assay, and total protein and albumin concentrations were determined. Pedigrees were evaluated to identify close relatives that had protein-losing enteropathy (PLE) or protein-losing nephropathy (PLN).

Results—39 of 188 (20.7%) dogs, including young dogs, had positive results for pANCA. Dogs had significantly higher odds of having positive results for pANCA if they had at least 1 littermate that had PLE or PLN (odds ratio, 12.1) or if they had at least 1 full sibling from another litter known to be affected with PLE or PLN (odds ratio, 4.0).

Conclusions and Clinical Relevance—This study revealed a high prevalence of pANCA in the serum of a representative sample of healthy SCWTs in the United Kingdom and a significant association between positive results for pANCA and a diagnosis of PLE or PLN in a sibling.

Abstract

Objective—To estimate the prevalence of perinuclear antineutrophilic cytoplasmic autoantibodies (pANCA) in the serum of healthy Soft Coated Wheaten Terriers (SCWTs) in the United Kingdom and to identify potential risk factors and heritability patterns associated with a positive result for pANCA.

Animals—188 SCWTs (age range, 18 months to 14.3 years).

Procedures—Blood samples were obtained from SCWTs in various locations in England. Serum was tested for pANCA by use of an immunofluorescence assay, and total protein and albumin concentrations were determined. Pedigrees were evaluated to identify close relatives that had protein-losing enteropathy (PLE) or protein-losing nephropathy (PLN).

Results—39 of 188 (20.7%) dogs, including young dogs, had positive results for pANCA. Dogs had significantly higher odds of having positive results for pANCA if they had at least 1 littermate that had PLE or PLN (odds ratio, 12.1) or if they had at least 1 full sibling from another litter known to be affected with PLE or PLN (odds ratio, 4.0).

Conclusions and Clinical Relevance—This study revealed a high prevalence of pANCA in the serum of a representative sample of healthy SCWTs in the United Kingdom and a significant association between positive results for pANCA and a diagnosis of PLE or PLN in a sibling.

Contributor Notes

Dr. Craig's present address is Discipline of Pharmacy, Faculty of Health, University of Canberra, Canberra, ACT 2601, Australia.

Supported by the UK Kennel Club Charitable Trust, the SCWT Club of Great Britain, and the Wheaten Health Initiative.

Presented in part as an oral presentation at the 18th European College of Veterinary Internal Medicine Conference, Ghent, Belgium, September 2008.

The authors thank Sandra Jeffries for assistance with collection of data from pedigree records.

Address correspondence to Dr. Wieland (bwieland@rvc.ac.uk).
Cover American Journal of Veterinary Research
American Journal of Veterinary Research
Publication Date:
01 Mar 2012
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