• 1.

    Werners AH, Bull S, Fink-Gremmels J. Endotoxaemia: a review with implications for the horse. Equine Vet J 2005; 37: 371383.

  • 2.

    Sykes BW, Furr MO. Equine endotoxaemia—a state-of-the-art review of therapy. Aust Vet J 2005; 83: 4550.

  • 3.

    Kelmer G. Update on treatments for endotoxemia. Vet Clin North Am Equine Pract 2009; 25: 259270.

  • 4.

    Bernard GR, Wheeler AP, Russell JA, et al. The effects of ibuprofen on the physiology and survival of patients with sepsis. The Ibuprofen in Sepsis Study Group. N Engl J Med 1997; 336: 912918.

    • Search Google Scholar
    • Export Citation
  • 5.

    MacAllister CG, Morgan SJ, Borne AT, et al. Comparison of adverse effects of phenylbutazone, flunixin meglumine, and ketoprofen in horses. J Am Vet Med Assoc 1993; 202: 7177.

    • Search Google Scholar
    • Export Citation
  • 6.

    Tomlinson JE, Blikslager AT. Effects of cyclooxygenase inhibitors flunixin and deracoxib on permeability of ischaemic-injured equine jejunum. Equine Vet J 2005; 37: 7580.

    • Search Google Scholar
    • Export Citation
  • 7.

    Barton MH, Parviainen A, Norton N. Polymyxin B protects horses against induced endotoxaemia in vivo. Equine Vet J 2004; 36: 397401.

  • 8.

    Raisbeck MF, Garner HE, Osweiler GD. Effects of polymyxin B on selected features of equine carbohydrate overload. Vet Hum Toxicol 1989; 31: 422426.

    • Search Google Scholar
    • Export Citation
  • 9.

    Gill AL, Bell CN. Hyperbaric oxygen: its uses, mechanisms of action and outcomes. Q J Med 2004; 97: 385395.

  • 10.

    Buras JA, Holt D, Orlow D, et al. Hyperbaric oxygen protects from sepsis mortality via an interleukin-10-dependent mechanism. Crit Care Med 2006; 34: 26242629.

    • Search Google Scholar
    • Export Citation
  • 11.

    Kudchodkar B, Jones H, Simecka J, et al. Hyperbaric oxygen treatment attenuates the pro-inflammatory and immune responses in apolipoprotein E knockout mice. Clin Immunol 2008; 128: 435441.

    • Search Google Scholar
    • Export Citation
  • 12.

    Niu KC, Lin MT, Kao CH. Prevention and suppression of pyrogenic fever in rabbits by hyperbaric oxygen. Eur J Pharmacol 2007; 570: 229234.

    • Search Google Scholar
    • Export Citation
  • 13.

    Lin HC, Wan FJ, Wu CC, et al. Hyperbaric oxygen protects against lipopolysaccharide-stimulated oxidative stress and mortality in rats. Eur J Pharmacol 2005; 508: 249254.

    • Search Google Scholar
    • Export Citation
  • 14.

    Pedoto A, Nandi J, Yang ZJ, et al. Beneficial effect of hyperbaric oxygen pretreatment on lipopolysaccharide-induced shock in rats. Clin Exp Pharmacol Physiol 2003; 30: 482488.

    • Search Google Scholar
    • Export Citation
  • 15.

    Huang TY, Tsai PS, Wang TY, et al. Hyperbaric oxygen attenuation of lipopolysaccharide-induced acute lung injury involves heme oxygenase-1. Acta Anaesthesiol Scand 2005; 49: 12931301.

    • Search Google Scholar
    • Export Citation
  • 16.

    Chang KY, Tsai PS, Huang TY, et al. HO-1 mediates the effects of HBO pretreatment against sepsis. J Surg Res 2006; 136: 143153.

  • 17.

    Slovis NM. Review of hyperbaric medicine, in Proceedings. 51st Annu Conv Am Assoc Equine Pract 2005; 153157.

  • 18.

    Moore JN, Norton N, Barton MH, et al. Rapid infusion of a phospholipid emulsion attenuates the effects of endotoxaemia in horses. Equine Vet J 2007; 39: 243248.

    • Search Google Scholar
    • Export Citation
  • 19.

    Winchell WW, Hardy J, Levine DM, et al. Effect of administration of a phospholipid emulsion on the initial response of horses administered endotoxin. Am J Vet Res 2002; 63: 13701378.

    • Search Google Scholar
    • Export Citation
  • 20.

    Kelmer G, Doherty TJ, Elliott S, et al. Evaluation of dimethyl sulphoxide effects on initial response to endotoxin in the horse. Equine Vet J 2008; 40: 358363.

    • Search Google Scholar
    • Export Citation
  • 21.

    Stadler P, van Amstel SR. Clinico-pathological changes after intravenous administration of endotoxin in the horse [in Afrikaans]. J S Afr Vet Assoc 1989; 60: 201205.

    • Search Google Scholar
    • Export Citation
  • 22.

    Morris DD, Crowe N, Moore JN. Correlation of clinical and laboratory data with serum tumor necrosis factor activity in horses with experimentally induced endotoxemia. Am J Vet Res 1990; 51: 19351940.

    • Search Google Scholar
    • Export Citation
  • 23.

    Le J, Vilcek J. Biology of disease; tumor necrosis factor and interleukin 1: cytokines with multiple overlapping biological activities. Lab Invest 1987; 56: 234248.

    • Search Google Scholar
    • Export Citation
  • 24.

    Fessler JF, Bottoms GD, Roesel OF, et al. Endotoxin-induced change in hemograms, plasma enzymes, and blood chemical values in anesthetized ponies: effects of flunixin meglumine. Am J Vet Res 1982; 43: 140144.

    • Search Google Scholar
    • Export Citation
  • 25.

    Ewert KM, Fessler JF, Templeton CB, et al. Endotoxin-induced hematologic and blood chemical changes in ponies: effects of flunixin meglumine, dexamethasone, and prednisolone. Am J Vet Res 1985; 46: 2430.

    • Search Google Scholar
    • Export Citation
  • 26.

    MacKay RJ, Daniels CA, Bleyaert HF, et al. Effect of eltenac in horses with induced endotoxaemia. Equine Vet J Suppl 2000;(32) 2631.

  • 27.

    Sprouse RF, Garner HE, Green EM. Plasma endotoxin levels in horses subjected to carbohydrate induced laminitis. Equine Vet J 1987; 19: 2528.

    • Search Google Scholar
    • Export Citation
  • 28.

    Pantaleon LG, Furr MO, McKenzie HC II, et al. Cardiovascular and pulmonary effects of hetastarch plus hypertonic saline solutions during experimental endotoxemia in anesthetized horses. J Vet Intern Med 2006; 20: 14221428.

    • Search Google Scholar
    • Export Citation
  • 29.

    Johnston K, Holcombe SJ, Hauptman JG. Plasma lactate as a predictor of colonic viability and survival after 360 degrees volvulus of the ascending colon in horses. Vet Surg 2007; 36: 563567.

    • Search Google Scholar
    • Export Citation
  • 30.

    Sueblinvong T, Egtasaeng N, Sanguangrangsirikul S. Hyperbaric oxygenation and blood lactate clearance: study in sixty male naval cadets. J Med Assoc Thai 2004; 87 (suppl 2): S218S222.

    • Search Google Scholar
    • Export Citation
  • 31.

    Weglicki WB, Whalen RE, Thompson HK Jr, et al. Effects of hyperbaric oxygenation on excess lactate production in exercising dogs. Am J Physiol 1966; 210: 473477.

    • Search Google Scholar
    • Export Citation
  • 32.

    Dinarello CA, Cannon JG, Wolff SM, et al. Tumor necrosis factor (cachectin) is an endogenous pyrogen and induces production of interleukin 1. J Exp Med 1986; 163: 14331450.

    • Search Google Scholar
    • Export Citation
  • 33.

    Simpson D. Hyperbaric oxygen therapy part one: the theory behind the practice. Aust Vet Pract 2009; 39: 93101.

  • 34.

    Lenhardt R, Kurz A, Sessler DI. Thermoregulation and hyperthermia. Acta Anaesthesiol Scand Suppl 1996;(109): 3438.

  • 35.

    Holowatz LA, Kenney WL. Peripheral mechanisms of thermoregulatory control of skin blood flow in aged humans. J Appl Physiol 2010; 109: 15381544.

    • Search Google Scholar
    • Export Citation
  • 36.

    Takayama K, Yuhki K, Ono K, et al. Thromboxane A2 and prostaglandin F2α mediate inflammatory tachycardia. Nat Med 2005; 11: 562566.

  • 37.

    King JN, Gerring EL. Detection of endotoxin in cases of equine colic. Vet Rec 1988; 123: 269271.

  • 38.

    Doherty TJ, Andrews FM, Abraha TW, et al. Metoclopramide ameliorates the effects of endotoxin on gastric emptying of acetaminophen in horses. Can J Vet Res 1999; 63: 3740.

    • Search Google Scholar
    • Export Citation
  • 39.

    Valk N, Doherty TJ, Blackford JT, et al. Phenylbutazone prevents the endotoxin-induced delay in gastric emptying in horses. Can J Vet Res 1998; 62: 214217.

    • Search Google Scholar
    • Export Citation
  • 40.

    Doherty TJ, Andrews FM, Blackford JT, et al. Effects of lipopolysaccharide and phenylbutazone on gastric contents in the horse. Equine Vet J 2003; 35: 472475.

    • Search Google Scholar
    • Export Citation
  • 41.

    Meisler SD, Doherty TJ, Andrews FM, et al. Yohimbine ameliorates the effects of endotoxin on gastric emptying of the liquid marker acetaminophen in horses. Can J Vet Res 2000; 64: 208211.

    • Search Google Scholar
    • Export Citation
  • 42.

    Lahat N, Bitterman H, Yaniv N, et al. Exposure to hyperbaric oxygen induces tumour necrosis factor-alpha (TNF-alpha) secretion from rat macrophages. Clin Exp Immunol 1995; 102: 655659.

    • Search Google Scholar
    • Export Citation
  • 43.

    Lin HC, Wan FJ. Hyperbaric oxygenation reduces overexpression of c-Fos and oxidative stress in the brain stem of experimental endotoxemic rats. Intensive Care Med 2008; 34: 11221132.

    • Search Google Scholar
    • Export Citation
  • 44.

    Chu SJ, Li MH, Hsu CW, et al. Influence of hyperbaric oxygen on tumor necrosis factor-alpha and nitric oxide production in endotoxin-induced acute lung injury in rats. Pulm Pharmacol Ther 2007; 20: 684690.

    • Search Google Scholar
    • Export Citation
  • 45.

    Holder TE, Schumacher J, Donnell RL, et al. Effects of hyperbaric oxygen on full-thickness meshed sheet skin grafts applied to fresh and granulating wounds in horses. Am J Vet Res 2008; 69: 144147.

    • Search Google Scholar
    • Export Citation
  • 46.

    Khan M, Meduru S, Mohan IK, et al. Hyperbaric oxygenation enhances transplanted cell graft and functional recovery in the infarct heart. J Mol Cell Cardiol 2009; 47: 275287.

    • Search Google Scholar
    • Export Citation
  • 47.

    Opal SM & Yu RL Jr. Antiendotoxin strategies for the prevention and treatment of septic shock. New approaches and future directions. Drugs 1998; 55: 497508.

    • Search Google Scholar
    • Export Citation
  • 48.

    West MA, Heagy W. Endotoxin tolerance: a review. Crit Care Med 2002; 30: S64S73.

  • 49.

    Hardie EM, Kruse-Elliott K. Endotoxic shock. Part II: a review of treatment. J Vet Intern Med 1990; 4: 306314.

  • 50.

    Allen GK, Campbell-Beggs C, Robinson JA, et al. Induction of early-phase endotoxin tolerance in horses. Equine Vet J 1996; 28: 269274.

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Effects of hyperbaric oxygen treatment on horses with experimentally induced endotoxemia

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  • 1 Departments of Large Animal Clinical Sciences, University of Tennessee, Knoxville, TN 37996.
  • | 2 Departments of Large Animal Clinical Sciences, University of Tennessee, Knoxville, TN 37996.
  • | 3 Departments of Large Animal Clinical Sciences, University of Tennessee, Knoxville, TN 37996.
  • | 4 College of Veterinary Medicine, University of Tennessee, Knoxville, TN 37996.
  • | 5 Departments of Large Animal Clinical Sciences, University of Tennessee, Knoxville, TN 37996.
  • | 6 Comparative Medicine, University of Tennessee, Knoxville, TN 37996.

Abstract

Objective—To determine the effectiveness of preinduction hyperbaric oxygen treatment (HBOT) in ameliorating signs of experimentally induced endotoxemia in horses.

Animals—18 healthy adult horses.

Procedures—Horses were randomly assigned to 1 of 3 equal-sized treatment groups to receive normobaric ambient air and lipopolysaccharide (LPS), HBOT and LPS, or HBOT and physiologic saline (0.9% NaCl) solution. Horses were physically examined, and blood was obtained for a CBC and to determine concentration or activity of plasma tissue necrosis factor-α, blood lactate, and blood glucose before the horses were treated with HBOT and then intermittently for 6 hours after administration of LPS or physiologic saline solution.

Results—All LPS-treated horses developed signs and biochemical and hematologic changes consistent with endotoxemia. Treatment with HBOT significantly ameliorated the effect of LPS on clinical endotoxemia score but did not significantly improve other abnormalities associated with endotoxemia.

Conclusions and Clinical Relevance—The protective effect of HBOT was minimal, and results did not support its use as a treatment for horses prior to development of endotoxemia.

Contributor Notes

Ms. Willis was a veterinary student at the time of this study. Ms. Willis's present address is 207 Crosswinds Dr, Chesapeake, VA 23320.

Supported by the Tennessee Equine Veterinary Research Organization.

The authors thank Anik Vasington, Sarah Elliot, and Cristina Catasus for technical support.

Address correspondence to Dr. Doherty (tdoherty@utk.edu).