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Composition and electrophoretic mobility of plasma lipoproteins of dromedary camels (Camelus dromedarius)

Farzad Asadi DVM, PhD1, Ali Shahriari DVM, PhD2, Peyman Asadian DVM, PhD3, Malihe Pourkabir DVM, PhD4, and Mahzyar Samadaei DVM5
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  • 1 Department of Biochemistry, School of Veterinary Medicine, University of Tehran, 1419963111 Tehran, Iran.
  • | 2 Department of Biochemistry, School of Veterinary Medicine, Shahid Chamran University, 6136668694 Ahvaz, Iran.
  • | 3 Department of Biochemistry, School of Veterinary Medicine, University of Tehran, 1419963111 Tehran, Iran.
  • | 4 Department of Biochemistry, School of Veterinary Medicine, University of Tehran, 1419963111 Tehran, Iran.
  • | 5 Private Veterinary Clinic of the Veterinary Organization, Province of Mazandaran, Ghaemshahr, Iran.

Abstract

Objective—To determine the lipid composition and electrophoretic pattern of plasma lipoproteins in samples obtained from healthy 1-humped camels (Camelus dromedarius).

Animals—34 healthy camels raised under similar farming and dietary conditions.

Procedures—Plasma samples were subjected to density-gradient ultracentrifugation for separation of plasma lipoproteins, including very-low-density lipoproteins (VLDL), low-density lipoproteins (LDL), and high-density lipoproteins (HDL). Purity of the separation was assessed by use of polyacrylamide gel disk electrophoresis. Concentrations of triglycerides, cholesterol, and phospholipids were measured in each lipoprotein fraction, and lipoprotein electrophoretic patterns were determined in plasma samples.

Results—Phospholipid was the major constituent of VLDL (mean ± SD concentration, 10.62 ± 1.2 mg/dL), LDL (24.66 ± 3.12 mg/dL), and HDL (38.08 ± 0.76 mg/dL). Low-density lipoprotein, VLDL, and HDL were important plasma lipoprotein carriers for cholesterol (67.94 ± 9.51%), triglyceride (55.83 ± 7.81%), and phospholipid (51.91 ± 1.55%), respectively. On the basis of electrophoresis results, relative percentages of α- and β-lipoproteins were 31.72 ± 4.88% and 68.3 ± 4.68%, respectively.

Conclusions and Clinical Relevance—The lipoprotein profile in 1-humped camels differed substantially from that of other ruminants. Results may be useful in the evaluation of metabolic disorders in camels.

Contributor Notes

The authors thank Dr. A. Golestani for providing technical equipment and H. Boshehri, F. Jadidizadeh, A. Naderloo, and Dr. Karen Lee for technical assistance.

Address correspondence to Dr. Asadi.