Determination of reference intervals for plasma biochemical values in clinically normal adult domestic shorthair cats by use of a dry-slide biochemical analyzer

Brice S. Reynolds Department of Clinical Sciences, National Veterinary School of Toulouse, 23, chemin des Capelles, BP 87614, 31076 Toulouse cedex 03, France.

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Karine G. Boudet Department of Clinical Sciences, National Veterinary School of Toulouse, 23, chemin des Capelles, BP 87614, 31076 Toulouse cedex 03, France.
Unité Mixte de Recherche 181 Physiopathologie et Toxicologie expérimentales, Institut National de la Recherche Agronomique, National Veterinary School of Toulouse, 23, chemin des Capelles, BP 87614, 31076 Toulouse cedex 03, France.

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Claude A. Germain Department of Clinical Sciences, National Veterinary School of Toulouse, 23, chemin des Capelles, BP 87614, 31076 Toulouse cedex 03, France.
Unité Mixte de Recherche 181 Physiopathologie et Toxicologie expérimentales, Institut National de la Recherche Agronomique, National Veterinary School of Toulouse, 23, chemin des Capelles, BP 87614, 31076 Toulouse cedex 03, France.

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Jean-Pierre D. Braun Department of Clinical Sciences, National Veterinary School of Toulouse, 23, chemin des Capelles, BP 87614, 31076 Toulouse cedex 03, France.
Unité Mixte de Recherche 181 Physiopathologie et Toxicologie expérimentales, Institut National de la Recherche Agronomique, National Veterinary School of Toulouse, 23, chemin des Capelles, BP 87614, 31076 Toulouse cedex 03, France.

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Herve P. Lefebvre Department of Clinical Sciences, National Veterinary School of Toulouse, 23, chemin des Capelles, BP 87614, 31076 Toulouse cedex 03, France.
Unité Mixte de Recherche 181 Physiopathologie et Toxicologie expérimentales, Institut National de la Recherche Agronomique, National Veterinary School of Toulouse, 23, chemin des Capelles, BP 87614, 31076 Toulouse cedex 03, France.

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Abstract

Objective—To establish reference intervals of plasma biochemical values in healthy adult domestic shorthair (DSH) cats by use of controlled conditions.

Animals—95 healthy client-owned cats.

Procedures—Food was withheld from the cats overnight. All blood samples were obtained on the same day, at the same location, and by the same investigator. Blood samples were collected from a cephalic vein into lithium heparin tubes. After centrifugation of blood samples, plasma supernatants were harvested and stored at −20°C until assayed for total proteins, albumin, creatinine, urea, glucose, calcium, phosphates, sodium, chloride, potassium, and CO2 concentrations and alkaline phosphatase and alanine aminotransferase activities.

Results—Reference intervals in healthy adult DSH cats were 65 to 85 g/L for total proteins, 27 to 39 g/L for albumin, 89 to 207 μmol/L for creatinine, 6.6 to 11.3 mmol/L for urea, 4.1 to 8.2 mmol/L for glucose, 2.4 to 2.9 mmol/L for calcium, 1.1 to 2.1 mmol/L for phosphates, 153 to 161 mmol/L for sodium, 120 to 127 mmol/L for chloride, 3.3 to 4.2 mmol/L for potassium, 15 to 21 mmol/L for CO2, 32 to 147 U/L for alkaline phosphatase, and 34 to 123 U/L for alanine aminotransferase.

Conclusions and Clinical Relevance—This study provided reference intervals for plasma analytes in adult DSH cats. The influence of potential confounding factors was minimized through use of controlled preanalytic and analytic conditions. However, these results cannot be extrapolated to other feline breeds or used to interpret results from other biochemical analyzers.

Abstract

Objective—To establish reference intervals of plasma biochemical values in healthy adult domestic shorthair (DSH) cats by use of controlled conditions.

Animals—95 healthy client-owned cats.

Procedures—Food was withheld from the cats overnight. All blood samples were obtained on the same day, at the same location, and by the same investigator. Blood samples were collected from a cephalic vein into lithium heparin tubes. After centrifugation of blood samples, plasma supernatants were harvested and stored at −20°C until assayed for total proteins, albumin, creatinine, urea, glucose, calcium, phosphates, sodium, chloride, potassium, and CO2 concentrations and alkaline phosphatase and alanine aminotransferase activities.

Results—Reference intervals in healthy adult DSH cats were 65 to 85 g/L for total proteins, 27 to 39 g/L for albumin, 89 to 207 μmol/L for creatinine, 6.6 to 11.3 mmol/L for urea, 4.1 to 8.2 mmol/L for glucose, 2.4 to 2.9 mmol/L for calcium, 1.1 to 2.1 mmol/L for phosphates, 153 to 161 mmol/L for sodium, 120 to 127 mmol/L for chloride, 3.3 to 4.2 mmol/L for potassium, 15 to 21 mmol/L for CO2, 32 to 147 U/L for alkaline phosphatase, and 34 to 123 U/L for alanine aminotransferase.

Conclusions and Clinical Relevance—This study provided reference intervals for plasma analytes in adult DSH cats. The influence of potential confounding factors was minimized through use of controlled preanalytic and analytic conditions. However, these results cannot be extrapolated to other feline breeds or used to interpret results from other biochemical analyzers.

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