Editorial Type:
Surgery

Evaluation of a laparoscopic technique for collection of serial full-thickness small intestinal biopsy specimens in standing sedated horses

José L. Bracamonte Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, Canada.

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 DVM
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Ludovic P. Bouré Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, Canada.

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 DVM, MSc
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Ray J. Geor Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, Canada.

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 BVSc, MVSc, PhD
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John R. Runciman School of Engineering, College of Physical & Engineering Science, University of Guelph, Guelph, ON N1G 2W1, Canada.

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Stephanie G. Nykamp Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, Canada.

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Antonio M. Cruz Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, Canada.

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Matthew G. Teeter Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, Canada.

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Heather L. Waterfall Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, Canada.

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DOI:
https://doi.org/10.2460/ajvr.69.3.431
Volume/Issue:
Volume 69: Issue 3
Online Publication Date:
01 Mar 2008
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Abstract

Objective—To assess a technique for laparoscopic collection of serial full-thickness small intestinal biopsy specimens in horses.

Animals—13 healthy adult horses.

Procedures—In the ex vivo portion of the study, sections of duodenum and jejunum obtained from 6 horses immediately after euthanasia were divided into 3 segments. Each segment was randomly assigned to the control group, the double-layer hand-sewn closure group, or the endoscopic linear stapler (ELS) group. Bursting strength and bursting wall tension were measured and compared among groups; luminal diameter reduction at the biopsy site was compared between the biopsy groups. In the in vivo portion of the study, serial full-thickness small intestinal biopsy specimens were laparoscopically collected with an ELS from the descending duodenum and distal portion of the jejunum at monthly intervals in 7 sedated, standing horses. Biopsy specimens were evaluated for suitability for histologic examination.

Results—Mean bursting strength and bursting wall tension were significantly lower in the ELS group than in the hand-sewn and control groups in both the duodenal and jejunal segments. Use of the hand-sewn closure technique at the biopsy site reduced luminal diameter significantly more than use of the stapling technique. In the in vivo part of the study, all 52 biopsy specimens collected during 26 laparoscopic procedures were suitable for histologic examination and no clinically important perioperative complications developed.

Conclusions and Clinical Relevance—Laparoscopic collection of serial full-thickness small intestinal biopsy specimens with a 45-mm ELS may be an effective and safe technique for use in healthy adult experimental horses.

Abstract

Objective—To assess a technique for laparoscopic collection of serial full-thickness small intestinal biopsy specimens in horses.

Animals—13 healthy adult horses.

Procedures—In the ex vivo portion of the study, sections of duodenum and jejunum obtained from 6 horses immediately after euthanasia were divided into 3 segments. Each segment was randomly assigned to the control group, the double-layer hand-sewn closure group, or the endoscopic linear stapler (ELS) group. Bursting strength and bursting wall tension were measured and compared among groups; luminal diameter reduction at the biopsy site was compared between the biopsy groups. In the in vivo portion of the study, serial full-thickness small intestinal biopsy specimens were laparoscopically collected with an ELS from the descending duodenum and distal portion of the jejunum at monthly intervals in 7 sedated, standing horses. Biopsy specimens were evaluated for suitability for histologic examination.

Results—Mean bursting strength and bursting wall tension were significantly lower in the ELS group than in the hand-sewn and control groups in both the duodenal and jejunal segments. Use of the hand-sewn closure technique at the biopsy site reduced luminal diameter significantly more than use of the stapling technique. In the in vivo part of the study, all 52 biopsy specimens collected during 26 laparoscopic procedures were suitable for histologic examination and no clinically important perioperative complications developed.

Conclusions and Clinical Relevance—Laparoscopic collection of serial full-thickness small intestinal biopsy specimens with a 45-mm ELS may be an effective and safe technique for use in healthy adult experimental horses.

Contributor Notes

Dr. Bouré's present address is AO Research Institute, Clavadelerstrasse 8, CH-7270 Davos Platz, Switzerland. Dr. Geor's present address is Middleburg Agricultural Research and Extension Center, Virginia Tech, 5527 Sullivans Mill Rd, Middleburg, VA 20117.

This manuscript represents a portion of a thesis by the senior author to the graduate school of the University of Guelph in partial fulfillment of requirements for the Doctor of Veterinary Science degree.

Supported by the Ontario Ministry of Agriculture and Food (grant No. 047889) and Ethicon EndoSurgery, Cincinnati, Ohio.

Presented at the Annual Scientific Meeting of the European College of Veterinary Surgeons, Seville, Spain, June 2006, and at the Resident Forum of the American College of Veterinary Surgeons Veterinary Symposium, Washington, DC, October 2006.

Address correspondence to Dr. Bouré.
Cover American Journal of Veterinary Research
American Journal of Veterinary Research
Publication Date:
01 Mar 2008
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