Editorial Type:
Clinical Pathology

In vitro evaluation of the effect of a novel immunosuppressive agent, FTY720, on the function of feline neutrophils

Yi-Je Chen Comparative Transplantation Laboratory, Department of Surgical and Radiological Sciences and the Center for Companion Animal Health, School of Veterinary Medicine, University of California, Davis, CA 95616-8745.

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 BVM, MS
,
Andrew E. Kyles Comparative Transplantation Laboratory, Department of Surgical and Radiological Sciences and the Center for Companion Animal Health, School of Veterinary Medicine, University of California, Davis, CA 95616-8745.

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 BVMS, PhD
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Clare R. Gregory Comparative Transplantation Laboratory, Department of Surgical and Radiological Sciences and the Center for Companion Animal Health, School of Veterinary Medicine, University of California, Davis, CA 95616-8745.

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 DVM
DOI:
https://doi.org/10.2460/ajvr.67.4.588
Volume/Issue:
Volume 67: Issue 4
Online Publication Date:
01 Apr 2006
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Abstract

Objective—To use in vitro assays to evaluate the effects of a novel immunosuppressive agent, FTY720, on biological functions (migration, phagocytosis, and production of reactive-oxygen species [ROS]) of feline peripheral neutrophils and determine the cytotoxic effects of FTY720 on feline peripheral neutrophils.

Sample Population—Peripheral neutrophils obtained from 8 healthy cats.

Procedure—Peripheral neutrophils were isolated from blood samples obtained from the 8 cats and exposed to the phosphorylated form of FTY720 (FTY720-P). A fluorescence-based in vitro evaluation of migration was performed. Phagocytosis of microbes and production of ROS were evaluated by use of a 2-color flow cytometry system. Samples of whole blood obtained from the cats were incubated with various concentrations of FTY720-P, fluorescein-labeled Staphylococcus aureus, and dihydroethidium. Cytotoxic effects were evaluated by use of propidium iodide staining.

Results—Addition of FTY720-P caused a slight non-significant decrease in phagocytosis and production of ROS by feline peripheral neutrophils. Migration activity of feline peripheral neutrophils was significantly increased by the addition of FTY720-P. Addition of FTY720-P at concentrations considered for clinical use did not increase the death rate of feline peripheral neutrophils.

Conclusions and Clinical Relevance—FTY720 does not inhibit critical functions of feline peripheral neutrophils in vitro.

Abstract

Objective—To use in vitro assays to evaluate the effects of a novel immunosuppressive agent, FTY720, on biological functions (migration, phagocytosis, and production of reactive-oxygen species [ROS]) of feline peripheral neutrophils and determine the cytotoxic effects of FTY720 on feline peripheral neutrophils.

Sample Population—Peripheral neutrophils obtained from 8 healthy cats.

Procedure—Peripheral neutrophils were isolated from blood samples obtained from the 8 cats and exposed to the phosphorylated form of FTY720 (FTY720-P). A fluorescence-based in vitro evaluation of migration was performed. Phagocytosis of microbes and production of ROS were evaluated by use of a 2-color flow cytometry system. Samples of whole blood obtained from the cats were incubated with various concentrations of FTY720-P, fluorescein-labeled Staphylococcus aureus, and dihydroethidium. Cytotoxic effects were evaluated by use of propidium iodide staining.

Results—Addition of FTY720-P caused a slight non-significant decrease in phagocytosis and production of ROS by feline peripheral neutrophils. Migration activity of feline peripheral neutrophils was significantly increased by the addition of FTY720-P. Addition of FTY720-P at concentrations considered for clinical use did not increase the death rate of feline peripheral neutrophils.

Conclusions and Clinical Relevance—FTY720 does not inhibit critical functions of feline peripheral neutrophils in vitro.

Contributor Notes

Presented in part at the American College of Veterinary Surgeons Veterinary Symposium, San Diego, October 2005.

Dr. Chen.
Cover American Journal of Veterinary Research
American Journal of Veterinary Research
Publication Date:
01 Apr 2006
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