Effects of ultraviolet radiation on 25-hydroxyvitamin D3 synthesis in red-eared slider turtles (Trachemys scripta elegans)

Mark J. Acierno Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70810.

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Mark A. Mitchell Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70810.

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Marlana K. Roundtree Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70810.

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Trevor T. Zachariah Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70810.

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Abstract

Objective—To determine whether there are increased concentrations of 25-hydroxyvitaminn D3 in red-eared slider turtles (Trachemys scripta elegans) after exposure to UV radiation.

Animals—12 yearling turtles recently removed from aestivation.

Procedures—Turtles were randomly allocated to 2 groups (6 turtles/group). An initial blood sample was collected from all turtles for measurement of 25-hydroxyvitamin D3 concentrations. Turtles of 1 group were then provided no supplemental lighting, whereas turtles of the other group were exposed to full-spectrum coil bulbs at a distance of 22.86 cm. The UV-A and UV-B radiation generated by the supplemental lighting was measured by use of a radiometer-photometer at weekly intervals. Measurements were collected 2.54 and 22.86 cm from the bulb surface. The study was continued for a 4-week period. At the end of the study, a second blood sample was collected from all turtles for measurement of 25-hydroxyvitamin D3.

Results—Mean ± SD 25-hydroxyvitamin D3 concentrations differed significantly between turtles provided supplemental UV radiation (71.7 ± 46.9 nmol/L) and those not provided UV radiation (31.4 ± 13.2 nmol/L).

Conclusions and Clinical Relevance—Appropriate husbandry recommendations for raising and maintaining red-eared slider turtles should include use of sunlight that is unobstructed by UV-B filtering material or provision of an artificial source of UV-B radiation.

Abstract

Objective—To determine whether there are increased concentrations of 25-hydroxyvitaminn D3 in red-eared slider turtles (Trachemys scripta elegans) after exposure to UV radiation.

Animals—12 yearling turtles recently removed from aestivation.

Procedures—Turtles were randomly allocated to 2 groups (6 turtles/group). An initial blood sample was collected from all turtles for measurement of 25-hydroxyvitamin D3 concentrations. Turtles of 1 group were then provided no supplemental lighting, whereas turtles of the other group were exposed to full-spectrum coil bulbs at a distance of 22.86 cm. The UV-A and UV-B radiation generated by the supplemental lighting was measured by use of a radiometer-photometer at weekly intervals. Measurements were collected 2.54 and 22.86 cm from the bulb surface. The study was continued for a 4-week period. At the end of the study, a second blood sample was collected from all turtles for measurement of 25-hydroxyvitamin D3.

Results—Mean ± SD 25-hydroxyvitamin D3 concentrations differed significantly between turtles provided supplemental UV radiation (71.7 ± 46.9 nmol/L) and those not provided UV radiation (31.4 ± 13.2 nmol/L).

Conclusions and Clinical Relevance—Appropriate husbandry recommendations for raising and maintaining red-eared slider turtles should include use of sunlight that is unobstructed by UV-B filtering material or provision of an artificial source of UV-B radiation.

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