Editorial Type:
Molecular Biology

Detection of novel papillomaviruslike sequences in paraffin-embedded specimens of invasive and in situ squamous cell carcinomas from cats

Gilles Nespeca Clinic for Small Animal Internal Medicine, Dermatology Unit, Vetsuisse Faculty, University of Zürich, Winterthurerstrasse 260, CH 8057 Zurich, Switzerland

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 Med Vet
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Paula Grest Institute for Veterinary Pathology, Vetsuisse Faculty, University of Zürich, Winterthurerstrasse 260, CH 8057 Zurich, Switzerland.

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 DVM
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Wayne S. Rosenkrantz Animal Dermatology Clinic, 5610 Kearny Mesa Rd, Ste B, San Diego, CA 92111.

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Mathias Ackermann Virology Institute, Vetsuisse Faculty, University of Zürich, Winterthurerstrasse 260, CH 8057 Zurich, Switzerland.

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 DVM, PhD
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Claude Favrot Clinic for Small Animal Internal Medicine, Dermatology Unit, Vetsuisse Faculty, University of Zürich, Winterthurerstrasse 260, CH 8057 Zurich, Switzerland

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 DrVet, MS
DOI:
https://doi.org/10.2460/ajvr.67.12.2036
Volume/Issue:
Volume 67: Issue 12
Online Publication Date:
01 Dec 2006
Restricted access
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Abstract

Objective—To detect and partially characterize papillomavirus (PV) DNA in squamous cell carcinoma (SCC) tumor specimens from cats.

Sample Population—54 formalin-fixed paraffinembedded skin biopsy specimens were examined. Specimens originated from Bowenoid in situ SCC (BISC; n = 21), invasive SCC (22), and skin affected by miscellaneous nonneoplastic conditions (11).

Procedures—Samples from each tissue block underwent DNA extraction after deparaffinization, and PCR assays were performed. Two sets of primers derived from PV E1 were used. The first set of primers was designed for the narrow-range PCR assay and was able to generate amplification products of feline PV (FePV), canine oral PV, or closely related PVs. The second set of primers was selected for the broad-range PCR assay because of its ability to amplify DNA from 64 human PVs. Sequence analysis of each amplified DNA was performed.

Results—1 of the 21 specimens of BISC was positive for PV DNA on the basis of narrow-range PCR assay results, whereas all the other specimens (BISC, invasive SCC, and controls) had negative results for PV DNA. In contrast, 5 of 21 BISC specimens and 4 of 22 invasive SCC specimens were positive for PV DNA on the basis of broad-range PCR assay results. Sequence analysis revealed that only 1 specimen was infected by a virus closely related to classic FePV. In the 8 other specimens positive for PV DNA, DNA of unknown PVs was uncovered.

Conclusions and Clinical Relevance—Bowenoid in situ SCC and invasive SCC of cats may be associated with PVs of genetic diversity.

Abstract

Objective—To detect and partially characterize papillomavirus (PV) DNA in squamous cell carcinoma (SCC) tumor specimens from cats.

Sample Population—54 formalin-fixed paraffinembedded skin biopsy specimens were examined. Specimens originated from Bowenoid in situ SCC (BISC; n = 21), invasive SCC (22), and skin affected by miscellaneous nonneoplastic conditions (11).

Procedures—Samples from each tissue block underwent DNA extraction after deparaffinization, and PCR assays were performed. Two sets of primers derived from PV E1 were used. The first set of primers was designed for the narrow-range PCR assay and was able to generate amplification products of feline PV (FePV), canine oral PV, or closely related PVs. The second set of primers was selected for the broad-range PCR assay because of its ability to amplify DNA from 64 human PVs. Sequence analysis of each amplified DNA was performed.

Results—1 of the 21 specimens of BISC was positive for PV DNA on the basis of narrow-range PCR assay results, whereas all the other specimens (BISC, invasive SCC, and controls) had negative results for PV DNA. In contrast, 5 of 21 BISC specimens and 4 of 22 invasive SCC specimens were positive for PV DNA on the basis of broad-range PCR assay results. Sequence analysis revealed that only 1 specimen was infected by a virus closely related to classic FePV. In the 8 other specimens positive for PV DNA, DNA of unknown PVs was uncovered.

Conclusions and Clinical Relevance—Bowenoid in situ SCC and invasive SCC of cats may be associated with PVs of genetic diversity.

Contributor Notes

Supported by grants from the Waltham Foundation and the European Society of Veterinary Dermatology.

Address correspondence to Dr. Favrot
Cover American Journal of Veterinary Research
American Journal of Veterinary Research
Publication Date:
01 Dec 2006
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