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Distribution of mRNA that codes for subtypes of adrenergic receptors in the gastrointestinal tract of dairy cows

Mireille MeylanClinic for Ruminants, Department of Clinical Veterinary Medicine, Vetsuisse Faculty of Berne, Bremgartenstrasse 109a, PO Box 8466, 3001 Berne, Switzerland.

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Teodora M. GeorgievaClinic for Ruminants, Department of Clinical Veterinary Medicine, Vetsuisse Faculty of Berne, Bremgartenstrasse 109a, PO Box 8466, 3001 Berne, Switzerland.
Present address is the Department of Veterinary Physiology and Physiological Chemistry, Faculty of Veterinary Medicine, Thrakia University, 6000 Stara Zagora, Bulgaria.

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Martin ReistClinic for Ruminants, Department of Clinical Veterinary Medicine, Vetsuisse Faculty of Berne, Bremgartenstrasse 109a, PO Box 8466, 3001 Berne, Switzerland.
Present address is the Swiss Federal Veterinary Office, Monitoring, CH-3003 Berne, Switzerland.

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Jürg W. BlumDivision of Nutrition and Physiology, Institute of Animal Genetics, Nutrition and Physiology, Vetsuisse Faculty of Berne, Bremgartenstrasse 109a, PO Box 8466, 3001 Berne, Switzerland.

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Johannes MartigClinic for Ruminants, Department of Clinical Veterinary Medicine, Vetsuisse Faculty of Berne, Bremgartenstrasse 109a, PO Box 8466, 3001 Berne, Switzerland.

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Ivan P. GeorgievaClinic for Ruminants, Department of Clinical Veterinary Medicine, Vetsuisse Faculty of Berne, Bremgartenstrasse 109a, PO Box 8466, 3001 Berne, Switzerland.
Present address is the Department of Veterinary Physiology and Physiological Chemistry, Faculty of Veterinary Medicine, Thrakia University, 6000 Stara Zagora, Bulgaria.

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Adrian SteinerClinic for Ruminants, Department of Clinical Veterinary Medicine, Vetsuisse Faculty of Berne, Bremgartenstrasse 109a, PO Box 8466, 3001 Berne, Switzerland.

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Abstract

Objective—To describe the distribution of mRNA that codes for 9 subtypes of adrenergic receptors in the digestive tract of dairy cows.

Sample Population—Fresh full-thickness wall specimens from the abomasum (fundus, corpus, and antrum), ileum, cecum, proximal loop of ascending colon, and 4 locations of the spiral colon collected from 10 healthy cows at slaughter.

Procedure—Concentrations of mRNA that code for 9 subtypes of adrenergic receptors in the bovine gastrointestinal tract (α1A, α1B, α1D, α2AD, α2B, α2C, β1, β2, and β3) were measured by use of a quantitative realtime reverse transcription-polymerase chain reaction assay. Results were reported in relation to mRNA expression of the housekeeping gene glyceraldehyde phosphate dehydrogenase (GAPDH).

Results—Mean mRNA contents of adrenergic receptors in the bovine digestive tract were low (range, 0.00006% to 5.04% of GAPDH). Distribution of receptor subtypes was similar in all tissues, with lowest expression of α1D receptors, followed by α2B, α2C, β3, α1B, α1A, β1, and β2 in the abomasum, whereas α2AD and β2 in the intestines were highest. In comparison with the intestines, relative concentrations of mRNA for receptors β2 and β3 were significantly lower in the abomasum.

Conclusions and Clinical Relevance—Relative concentrations of mRNA that code for adrenergic receptors differed among receptor subtypes and among locations in the bovine gastrointestinal tract. Comparison of these values established in healthy cattle with results for cows with motility disorders, such as abomasal displacement and cecal dilatation, may lead to improved therapeutic or prophylactic approaches for these diseases. (Am J Vet Res 2004;65:1142–1150)

Abstract

Objective—To describe the distribution of mRNA that codes for 9 subtypes of adrenergic receptors in the digestive tract of dairy cows.

Sample Population—Fresh full-thickness wall specimens from the abomasum (fundus, corpus, and antrum), ileum, cecum, proximal loop of ascending colon, and 4 locations of the spiral colon collected from 10 healthy cows at slaughter.

Procedure—Concentrations of mRNA that code for 9 subtypes of adrenergic receptors in the bovine gastrointestinal tract (α1A, α1B, α1D, α2AD, α2B, α2C, β1, β2, and β3) were measured by use of a quantitative realtime reverse transcription-polymerase chain reaction assay. Results were reported in relation to mRNA expression of the housekeeping gene glyceraldehyde phosphate dehydrogenase (GAPDH).

Results—Mean mRNA contents of adrenergic receptors in the bovine digestive tract were low (range, 0.00006% to 5.04% of GAPDH). Distribution of receptor subtypes was similar in all tissues, with lowest expression of α1D receptors, followed by α2B, α2C, β3, α1B, α1A, β1, and β2 in the abomasum, whereas α2AD and β2 in the intestines were highest. In comparison with the intestines, relative concentrations of mRNA for receptors β2 and β3 were significantly lower in the abomasum.

Conclusions and Clinical Relevance—Relative concentrations of mRNA that code for adrenergic receptors differed among receptor subtypes and among locations in the bovine gastrointestinal tract. Comparison of these values established in healthy cattle with results for cows with motility disorders, such as abomasal displacement and cecal dilatation, may lead to improved therapeutic or prophylactic approaches for these diseases. (Am J Vet Res 2004;65:1142–1150)