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Evaluation of leukocyte cell surface markers in dogs with septic and nonseptic inflammatory diseases

Douglas J. Weiss DVM, PhD1,2, Molly Welle3, Andreas Mortiz Dr Med Vet4, and Bruce Walcheck PhD5
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  • 1 Department of Veterinary PathoBiology, College of Veterinary Medicine, University of Minnesota, St Paul, MN 55108.
  • | 2 Present address is the Department of Veterinary PathoBiology, College of Veterinary Medicine, University of Minnesota, 1971 Commonwealth Ave, St Paul, MN 55108.
  • | 3 Department of Veterinary PathoBiology, College of Veterinary Medicine, University of Minnesota, St Paul, MN 55108.
  • | 4 Justus-Liebig-University, Giessen, Germany.
  • | 5 Department of Veterinary PathoBiology, College of Veterinary Medicine, University of Minnesota, St Paul, MN 55108.

Abstract

Objective—To determine whether functional alterations in neutrophils and mononuclear leukocytes are a consistent finding in dogs with inflammatory disease.

Animals—40 healthy dogs, 30 dogs with nonseptic inflammatory diseases, 25 dogs with septic inflammation, and 8 dogs with multiple organ dysfunction syndrome (MODS) secondary to sepsis.

Procedure—Neutrophil size and granularity; expression of cell surface molecules including CD18, CD11b, and mature neutrophil antigen on neutrophils; and major histocompatability antigen class II (MHC class II) expression on monocytes and lymphocytes were evaluated by use of flow cytometry. Neutrophil size and granularity were evaluated by use of forwardangle versus side-angle light scatterplots. Leukocytes were labeled with monoclonal antibodies to quantify surface expression of leukocyte antigens.

Results—Dogs with septic and nonseptic inflammatory diseases and MODS had an increase in percentage of neutrophils with increased size; dogs with septic inflammation and MODS had a greater percentage of neutrophils with decreased granularity. Dogs with septic and nonseptic inflammation and MODS had a low expression of CD18 and mature neutrophil antigen. Dogs with septic and nonseptic inflammation had an increase in CD11b expression. Monocytes from dogs with septic and nonseptic inflammation and MODS had a low expression of CD18. Monocytes and lymphocytes from dogs with septic and nonseptic inflammation and MODS had a low expression of MHC class II.

Conclusions and Clinical Relevance—Neutrophils from dogs with septic and nonseptic inflammation circulate in an activated state, and some dogs have decreased MHC class II expression. Many dogs with MODS have a compensatory anti-inflammatory response that may compromise their responses to antimicrobials. ( Am J Vet Res 2004;65:59–63)

Abstract

Objective—To determine whether functional alterations in neutrophils and mononuclear leukocytes are a consistent finding in dogs with inflammatory disease.

Animals—40 healthy dogs, 30 dogs with nonseptic inflammatory diseases, 25 dogs with septic inflammation, and 8 dogs with multiple organ dysfunction syndrome (MODS) secondary to sepsis.

Procedure—Neutrophil size and granularity; expression of cell surface molecules including CD18, CD11b, and mature neutrophil antigen on neutrophils; and major histocompatability antigen class II (MHC class II) expression on monocytes and lymphocytes were evaluated by use of flow cytometry. Neutrophil size and granularity were evaluated by use of forwardangle versus side-angle light scatterplots. Leukocytes were labeled with monoclonal antibodies to quantify surface expression of leukocyte antigens.

Results—Dogs with septic and nonseptic inflammatory diseases and MODS had an increase in percentage of neutrophils with increased size; dogs with septic inflammation and MODS had a greater percentage of neutrophils with decreased granularity. Dogs with septic and nonseptic inflammation and MODS had a low expression of CD18 and mature neutrophil antigen. Dogs with septic and nonseptic inflammation had an increase in CD11b expression. Monocytes from dogs with septic and nonseptic inflammation and MODS had a low expression of CD18. Monocytes and lymphocytes from dogs with septic and nonseptic inflammation and MODS had a low expression of MHC class II.

Conclusions and Clinical Relevance—Neutrophils from dogs with septic and nonseptic inflammation circulate in an activated state, and some dogs have decreased MHC class II expression. Many dogs with MODS have a compensatory anti-inflammatory response that may compromise their responses to antimicrobials. ( Am J Vet Res 2004;65:59–63)