Response to Malassezia pachydermatis by peripheral blood mononuclear cells from clinically normal and atopic dogs
Search for other papers by Daniel O. Morris in
Current site
Google Scholar
PubMed
Search for other papers by Daniel J. Clayton in
Current site
Google Scholar
PubMed
Search for other papers by Kenneth J. Drobatz in
Current site
Google Scholar
PubMed
Search for other papers by Peter J. Felsburg in
Current site
Google Scholar
PubMed
Abstract
Objective–To investigate the potential cell-mediated immune response of atopic dogs to the yeast Malassezia pachydermatis and to correlate it with the type-1 hypersensitivity (humoral) response of the same population of dogs.
Animals–16 clinically normal dogs, 15 atopic dogs with Malassezia dermatitis, 5 atopic dogs with Malassezia otitis, and 7 atopic control (ie, without Malassezia dermatitis or otitis) dogs.
Procedure–A crude extract of M pachydermatis was extracted for use as an intradermal allergy testing reagent and for stimulation of isolated peripheral blood mononuclear cells in vitro. Flow cytometry was also used to assess cell surface antigenic determinants (CD3, CD4, CD8, CD14, CD21, CD45RA, surface immunoglobulin) on peripheral blood mononuclear cells.
Results–Atopic dogs with cytologic evidence of Malassezia dermatitis had an increased lymphocyte blastogenic response to crude M pachydermatis extract, compared with clinically normal dogs and dogs with Malassezia otitis. Atopic control dogs did not differ significantly in their responses from atopic dogs with Malassezia dermatitis or otitis. A significant correlation was not found between the lymphocyte blastogenic response and the type-1 hypersensitivity response to M pachydermatis within any of the groups.
Conclusions and Clinical Relevance–Cell-mediated and humoral reactivities to M pachydermatis contribute to the pathogenesis of atopic dermatitis in dogs but are not directly correlated. Modification of the dysregulated immune response toward M pachydermatis may assist in the reduction of pathologic changes associated with an atopic dermatitis phenotype in dogs. (Am J Vet Res 2002;63:358–362)
| All Time | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 54 | 0 | 0 |
| Full Text Views | 1475 | 1230 | 22 |
| PDF Downloads | 192 | 67 | 9 |
