Effect of caprine arthritis-encephalitis virus infection on expression of interleukin-16 in goats

C. Sharmila Department of Pathobiology, College of Veterinary Medicine, Tuskegee University, Tuskegee, AL 36088.

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 DVM, MS
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John W. Williams Department of Center for Biomedical Research, Tuskegee University, Tuskegee, AL 36088.

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P. Gopal Reddy Departments of Pathobiology, College of Veterinary Medicine, Center for Biomedical Research, Tuskegee University, Tuskegee, AL 36088.

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 DVM, PhD

Abstract

Objective—To determine the effect of caprine arthritis- encephalitis virus (CAEV) infection on expression of interleukin-16 (IL-16).

Animals—-6 goats experimentally infected with CAEV and 6 age-matched healthy uninfected control goats.

Procedure—-Peripheral blood mononuclear cells (PBMCs) and synovial membrane cells from infected and control goats cultured with or without phytohemagglutinin were analyzed for IL-16 mRNA by use of a reverse transcriptase-polymerase chain reaction assay with goat-specific primers, after cloning and sequencing of a 384-bp fragment of the goat IL-16 gene. Synovial fluid, serum, and culture supernatants of PBMCs and synovial cells of control and CAEVinfected goats were analyzed for IL-16 by use of an ELISA.

Results—-The 384-bp product was 86% homologous to the corresponding human IL-16 nucleotide sequence. Higher expression of IL-16 mRNA in PBMCs (unstimulated or stimulated with phytohemagglutinin) was detected in samples from CAEVinfected goats, compared with control goats, but the difference was not significant. Synovial membrane cells infected in vitro had higher expression than uninfected control cells. Higher IL-16 concentration was detected in synovial fluid, serum, and culture supernatants of PBMCs of infected goats than in samples from control goats.

Conclusions and Clinical Relevance—Infection with CAEV increases expression of IL-16, a proinflammatory and chemotactic cytokine. This cytokine appears to be constitutively expressed at low concentrations in normal uninfected PBMCs and synovial membrane cells. Increased production of IL-16 in CAEV infection may partly be responsible for increased lymphoid cell infiltrations observed in arthritic joints and other tissues of CAEV-infected goats. (Am J Vet Res 2002;63:1418–1422)

Abstract

Objective—To determine the effect of caprine arthritis- encephalitis virus (CAEV) infection on expression of interleukin-16 (IL-16).

Animals—-6 goats experimentally infected with CAEV and 6 age-matched healthy uninfected control goats.

Procedure—-Peripheral blood mononuclear cells (PBMCs) and synovial membrane cells from infected and control goats cultured with or without phytohemagglutinin were analyzed for IL-16 mRNA by use of a reverse transcriptase-polymerase chain reaction assay with goat-specific primers, after cloning and sequencing of a 384-bp fragment of the goat IL-16 gene. Synovial fluid, serum, and culture supernatants of PBMCs and synovial cells of control and CAEVinfected goats were analyzed for IL-16 by use of an ELISA.

Results—-The 384-bp product was 86% homologous to the corresponding human IL-16 nucleotide sequence. Higher expression of IL-16 mRNA in PBMCs (unstimulated or stimulated with phytohemagglutinin) was detected in samples from CAEVinfected goats, compared with control goats, but the difference was not significant. Synovial membrane cells infected in vitro had higher expression than uninfected control cells. Higher IL-16 concentration was detected in synovial fluid, serum, and culture supernatants of PBMCs of infected goats than in samples from control goats.

Conclusions and Clinical Relevance—Infection with CAEV increases expression of IL-16, a proinflammatory and chemotactic cytokine. This cytokine appears to be constitutively expressed at low concentrations in normal uninfected PBMCs and synovial membrane cells. Increased production of IL-16 in CAEV infection may partly be responsible for increased lymphoid cell infiltrations observed in arthritic joints and other tissues of CAEV-infected goats. (Am J Vet Res 2002;63:1418–1422)

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