Differentiation of Haemobartonella canis and Mycoplasma haemofelis on the basis of comparative analysis of gene sequences

Adam J. Birkenheuer Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606- 1428.

Search for other papers by Adam J. Birkenheuer in
Current site
Google Scholar
PubMed
Close
 DVM
,
Edward B. Breitschwerdt Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606- 1428.

Search for other papers by Edward B. Breitschwerdt in
Current site
Google Scholar
PubMed
Close
 DVM
,
A. Rick Alleman Department of Physiological Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610.

Search for other papers by A. Rick Alleman in
Current site
Google Scholar
PubMed
Close
 DVM, PhD
, and
Christian Pitulle Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606- 1428.

Search for other papers by Christian Pitulle in
Current site
Google Scholar
PubMed
Close
 PhD

Abstract

Objective—To determine whether Haemobartonella canis and Mycoplasma haemofelis (formerly known as H felis [large form]) can be differentiated by use of comparative analysis of gene sequences.

Sample Population—Blood samples obtained from 3 dogs infected with H canis and 2 cats infected with M haemofelis.

Procedure—The partial 16S rDNA and ribonuclease P RNA (RNase P) genes were amplified, cloned, and sequenced in blood samples obtained from H canis-infected dogs and M haemofelis-infected cats. The DNA sequences were subjected to comparative analysis.

Results—The 16S rDNA sequences of H canis and M haemofelis were nearly identical (homology of 99.3 to 99.7%). In contrast, RNase P gene sequences had a lower degree of sequence homology between the 2 organisms (94.3 to 95.5%).

Conclusions and Clinical RelevanceHaemobartonella canis and M haemofelis are not identical organisms. Molecular differentiation of H canis and M haemofelis is more clearly evident by use of comparative analysis of RNase P gene sequences than by comparative analysis of 16S rDNA gene sequences. (Am J Vet Res 2002;63:1385–1388)

Abstract

Objective—To determine whether Haemobartonella canis and Mycoplasma haemofelis (formerly known as H felis [large form]) can be differentiated by use of comparative analysis of gene sequences.

Sample Population—Blood samples obtained from 3 dogs infected with H canis and 2 cats infected with M haemofelis.

Procedure—The partial 16S rDNA and ribonuclease P RNA (RNase P) genes were amplified, cloned, and sequenced in blood samples obtained from H canis-infected dogs and M haemofelis-infected cats. The DNA sequences were subjected to comparative analysis.

Results—The 16S rDNA sequences of H canis and M haemofelis were nearly identical (homology of 99.3 to 99.7%). In contrast, RNase P gene sequences had a lower degree of sequence homology between the 2 organisms (94.3 to 95.5%).

Conclusions and Clinical RelevanceHaemobartonella canis and M haemofelis are not identical organisms. Molecular differentiation of H canis and M haemofelis is more clearly evident by use of comparative analysis of RNase P gene sequences than by comparative analysis of 16S rDNA gene sequences. (Am J Vet Res 2002;63:1385–1388)

Advertisement