Regional and zonal variations in the sulfation patterns of chondroitin sulfate in normal equine corneal stroma

Daniel J. Biros Department of Large and Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610.
Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, 20 Staniford St, Boston MA 02114.

Search for other papers by Daniel J. Biros in
Current site
Google Scholar
PubMed
Close
 DVM
,
Dennis E. Brooks Department of Large and Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610.

Search for other papers by Dennis E. Brooks in
Current site
Google Scholar
PubMed
Close
 DVM, PhD
,
Murray P. Brown Department of Large and Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610.

Search for other papers by Murray P. Brown in
Current site
Google Scholar
PubMed
Close
 DVM, MSc
,
Kelly A. Merritt Department of Large and Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610.

Search for other papers by Kelly A. Merritt in
Current site
Google Scholar
PubMed
Close
 BS
, and
Paul S. Kubilis Department of Large and Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610.

Search for other papers by Paul S. Kubilis in
Current site
Google Scholar
PubMed
Close
 MS

Abstract

Objective—To determine regional and zonal variation in sulfation patterns of chondroitin sulfate in normal equine corneal stroma.

Sample Population—22 normal eyes from 11 horses.

Procedure—Corneas were collected within 24 hours of death from equine necropsy specimens. After papain-chondroitinase digestion of corneal tissue, disaccharides ΔDi4S and ΔDi6S were quantified by use of capillary zone electrophoresis in the superficial, middle, and deep zones of central and peripheral regions of the cornea.

Results—For the 2 regions combined,ΔDi6S/ΔDi4S values were significantly lower in the deep and middle zones, compared with that of the superficial zone. In the central region, deep and middle zones had significantly lower ΔDi6S/ΔDi4S values than the superficial zone did. In the peripheral region, the deep zone had significantly lower ΔDi6S/ΔDi4S values, compared with superficial and middle zones. In the deep zone, the peripheral region had significantly lower ΔDi6S/ΔDi4S values than the central region did.

Conclusion and Clinical Relevance—Distribution of ΔDi6S/ΔDi4S values follows a gradient across the healthy equine cornea, being smallest in the deep and middle zones of the central region and the deep zone of the peripheral region. Regional and zonal differences in the distribution of stromal ΔDi6S and ΔDi4S may influence the role of glycosaminoglycans in health, disease, and wound repair of the equine cornea. (Am J Vet Res 2002;63:143–147)

Abstract

Objective—To determine regional and zonal variation in sulfation patterns of chondroitin sulfate in normal equine corneal stroma.

Sample Population—22 normal eyes from 11 horses.

Procedure—Corneas were collected within 24 hours of death from equine necropsy specimens. After papain-chondroitinase digestion of corneal tissue, disaccharides ΔDi4S and ΔDi6S were quantified by use of capillary zone electrophoresis in the superficial, middle, and deep zones of central and peripheral regions of the cornea.

Results—For the 2 regions combined,ΔDi6S/ΔDi4S values were significantly lower in the deep and middle zones, compared with that of the superficial zone. In the central region, deep and middle zones had significantly lower ΔDi6S/ΔDi4S values than the superficial zone did. In the peripheral region, the deep zone had significantly lower ΔDi6S/ΔDi4S values, compared with superficial and middle zones. In the deep zone, the peripheral region had significantly lower ΔDi6S/ΔDi4S values than the central region did.

Conclusion and Clinical Relevance—Distribution of ΔDi6S/ΔDi4S values follows a gradient across the healthy equine cornea, being smallest in the deep and middle zones of the central region and the deep zone of the peripheral region. Regional and zonal differences in the distribution of stromal ΔDi6S and ΔDi4S may influence the role of glycosaminoglycans in health, disease, and wound repair of the equine cornea. (Am J Vet Res 2002;63:143–147)

All Time Past Year Past 30 Days
Abstract Views 46 0 0
Full Text Views 1410 1250 62
PDF Downloads 67 31 2
Advertisement