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Characterization of Pasteurellaspp isolated from healthy domestic pack goats and evaluation of the effects of a commercial Pasteurella vaccine

Alton C. S. WardCollege of Agriculture, Caine Veterinary Teaching Center, University of Idaho, Caldwell, ID 83607.

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Glen C. WeiserCollege of Agriculture, Caine Veterinary Teaching Center, University of Idaho, Caldwell, ID 83607.

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Walter J. DeLongBureau of Laboratories, Idaho Department of Health and Welfare, 2220 Old Penitentiary Rd, Boise, ID.

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Glynn H. FrankNational Animal Disease Center, Agriculture Research Services, USDA, 2300 Dayton Rd, Ames, IA 50010.

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Abstract

Objective—To characterize Pasteurella spp isolated from healthy pack goats and evaluate the effects of administration of a commercial Pasteurellavaccine.

Animals—45 goats.

Procedure—Pharyngeal swab specimens and blood samples were collected on day 0 before vaccination with a Pasteurella (Mannheimia) haemolytica serotype A1 bacterin. Samples were also collected from 17 goats on days 21 and 35. Isolated Pasteurella spp were assigned to biovariant groups on the basis of results of biochemical utilization tests and serotyped. Serum antibody titers were determined.

Results—Multiple strains of Pasteurellaspp were isolated from swab specimens and assigned to 30 nonhemolytic and 14 β-hemolytic biovariant groups. The most common biovariant isolated was nonhemolytic P trehalosi belonging to group 2. This strain was isolated from 41 goats. Nonhemolytic P haemolytica strains were isolated from 31 goats, whereas β-hemolytic strains of P trehalosi and P haemolytica were isolated from 8 and 35 goats, respectively. Vaccination with the A1 serotype did not affect the proportion of goats from which we isolated each biovariant group or the number of biovariant groups isolated.

Conclusion and Clinical Relevance—Multiple strains of P haemolytica and P trehalosi belonging to nonhemolytic and β-hemolytic biovariant groups were isolated from the pharynx of healthy domestic pack goats. Because hemolytic activity correlates with leukotoxin production, β-hemolytic strains may have a greater potential to cause disease in naive populations of wild ruminants. However, vaccination with an A1 serotype bacterin did not decrease the proportion of culture-positive goats. (Am J Vet Res 2002;63:119–123)

Abstract

Objective—To characterize Pasteurella spp isolated from healthy pack goats and evaluate the effects of administration of a commercial Pasteurellavaccine.

Animals—45 goats.

Procedure—Pharyngeal swab specimens and blood samples were collected on day 0 before vaccination with a Pasteurella (Mannheimia) haemolytica serotype A1 bacterin. Samples were also collected from 17 goats on days 21 and 35. Isolated Pasteurella spp were assigned to biovariant groups on the basis of results of biochemical utilization tests and serotyped. Serum antibody titers were determined.

Results—Multiple strains of Pasteurellaspp were isolated from swab specimens and assigned to 30 nonhemolytic and 14 β-hemolytic biovariant groups. The most common biovariant isolated was nonhemolytic P trehalosi belonging to group 2. This strain was isolated from 41 goats. Nonhemolytic P haemolytica strains were isolated from 31 goats, whereas β-hemolytic strains of P trehalosi and P haemolytica were isolated from 8 and 35 goats, respectively. Vaccination with the A1 serotype did not affect the proportion of goats from which we isolated each biovariant group or the number of biovariant groups isolated.

Conclusion and Clinical Relevance—Multiple strains of P haemolytica and P trehalosi belonging to nonhemolytic and β-hemolytic biovariant groups were isolated from the pharynx of healthy domestic pack goats. Because hemolytic activity correlates with leukotoxin production, β-hemolytic strains may have a greater potential to cause disease in naive populations of wild ruminants. However, vaccination with an A1 serotype bacterin did not decrease the proportion of culture-positive goats. (Am J Vet Res 2002;63:119–123)