Objective—To determine effects of commonly used
ophthalmic antibiotics on cellular morphologic characteristics
and migration of canine corneal epithelium in
Sample Population—Corneal epithelial cells harvested
from corneas of 12 euthanatized dogs and propagated
in cell culture.
Procedure—Cells were treated with various antibiotics
after a defect was created in the monolayer.
Cellular morphologic characteristics and closure of
the defect were compared between antibiotic-treated
and control cells.
Results—Cells treated with ciprofloxacin and cefazolin
had the greatest degree of rounding, shrinkage,
and detachment from plates. Cells treated with
neomycin-polymyxin B-gramicidin and gentamicin sulfate
had rounding and shrinkage but with less
detachment. Cells treated with tobramycin and chloramphenicol
grew similarly to control cells. On the
basis of comparisons of defect circumference
between control cells and cells exposed to antibiotics,
tobramycin affected cellular migration the least.
Conclusion and Clinical Relevance—Effects of
ciprofloxacin and cefazolin on morphologic characteristics
of canine corneal epithelial cells in vitro should
be taken into consideration before using these antibiotics
for first-line of treatment for noninfected ulcers.
Of the antibiotics tested that have a primarily gramnegative
spectrum of coverage, gentamicin inhibited
corneal epithelial cell migration and had greater
cytopathologic effects than tobramycin did. For antibiotics
with a gram-positive coverage, chloramphenicol
had no cytopathologic effects on cells in comparison
to cefazolin, which caused most of the cells to shrink
and detach from the plate. Polymyxin B-neomycingramicidin
was midrange in its effects on cellular morphologic
characteristics and migration. (Am J Vet Res