Evaluation of a polyvalent enzyme-linked immunosorbent assay incorporating a recombinant p44 antigen for diagnosis of granulocytic ehrlichiosis in dogs and horses

Louis A. Magnarelli Department of Entomology, Connecticut Agricultural Experiment Station, New Haven, CT 06504 Yale University School of Medicine, New Haven, CT 06520.

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Jacob W. IJdo Section of Rheumatology, Department of Internal Medicine Yale University School of Medicine, New Haven, CT06520. .
College of Medicine, University of Iowa, Iowa City, IA 52242.

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Amy E. Van Andel Department of Epidemiology and Public Health Yale University School of Medicine, New Haven, CT06520.
Boehringer Ingelheim Pharmaceuticals Inc, 900 Ridgebury Rd, Ridgefield, CT 06877–0368.

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Caiyun Wu Section of Rheumatology, Department of Internal Medicine Yale University School of Medicine, New Haven, CT06520.

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Erol Fikrig Section of Rheumatology, Department of Internal Medicine Yale University School of Medicine, New Haven, CT06520.

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Abstract

Objective—To develop and evaluate a polyvalent ELISA incorporating a highly specific recombinant antigen (p44) for diagnosis of granulocytic ehrlichiosis in dogs and horses.

Animals—32 dogs and 43 horses.

Procedure—Results of the ELISA were compared with results of indirect fluorescent antibody (IFA) staining and western immunoblotting incorporating whole-cell antigen.

Results—For the canine and equine samples, percentages of samples with positive IFA staining, western immunoblotting, and ELISA results were similar. For 29 (91%) canine samples and 30 (70%) equine samples, results of IFA staining, western immunoblotting, and the ELISA were in complete agreement. Results of the ELISA for 3 canine serum samples known to contain antibodies to Ehrlichia canis and 12 equine serum samples known to contain antibodies to E risticii were negative.

Conclusions and Clinical Relevance—Results of the present study suggest that a polyvalent ELISA incorporating a recombinant p44 antigen is suitable for detecting antibodies to E equi in dogs and horses. ( Am J Vet Res 2001;62:29–32)

Abstract

Objective—To develop and evaluate a polyvalent ELISA incorporating a highly specific recombinant antigen (p44) for diagnosis of granulocytic ehrlichiosis in dogs and horses.

Animals—32 dogs and 43 horses.

Procedure—Results of the ELISA were compared with results of indirect fluorescent antibody (IFA) staining and western immunoblotting incorporating whole-cell antigen.

Results—For the canine and equine samples, percentages of samples with positive IFA staining, western immunoblotting, and ELISA results were similar. For 29 (91%) canine samples and 30 (70%) equine samples, results of IFA staining, western immunoblotting, and the ELISA were in complete agreement. Results of the ELISA for 3 canine serum samples known to contain antibodies to Ehrlichia canis and 12 equine serum samples known to contain antibodies to E risticii were negative.

Conclusions and Clinical Relevance—Results of the present study suggest that a polyvalent ELISA incorporating a recombinant p44 antigen is suitable for detecting antibodies to E equi in dogs and horses. ( Am J Vet Res 2001;62:29–32)

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