Advertisement

Effect of all-trans and 9-cis retinoic acid on growth and metastasis of xenotransplanted canine osteosarcoma cells in athymic mice

Sung-Hyeok HongPediatrics Oncology Branch, Division of Clinical Sciences, National Cancer Institute, 10/13N240 Center Dr, Bethesda, MD, 20892.
Laboratory of Veterinary Surgery, Division of Veterinary Medical Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan 113- 8657.

Search for other papers by Sung-Hyeok Hong in
Current site
Google Scholar
PubMed
Close
 DVM, PhD
,
Tsuyoshi KadosawaLaboratory of Veterinary Surgery, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan 060-0818.

Search for other papers by Tsuyoshi Kadosawa in
Current site
Google Scholar
PubMed
Close
 DVM, PhD
,
Manabu MochizukiLaboratory of Veterinary Surgery, Division of Veterinary Medical Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan 113- 8657.

Search for other papers by Manabu Mochizuki in
Current site
Google Scholar
PubMed
Close
 DVM, PhD
,
Satoru MatsunagaVeterinary Medical Center , The University of Tokyo, Japan 113- 8657.

Search for other papers by Satoru Matsunaga in
Current site
Google Scholar
PubMed
Close
 DVM
,
Ryohei NishimuraLaboratory of Veterinary Surgery, Division of Veterinary Medical Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan 113- 8657.

Search for other papers by Ryohei Nishimura in
Current site
Google Scholar
PubMed
Close
 DVM, PhD
, and
Nobuo SasakiLaboratory of Veterinary Surgery, Division of Veterinary Medical Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan 113- 8657.

Search for other papers by Nobuo Sasaki in
Current site
Google Scholar
PubMed
Close
 DVM, PhD

Abstract

Objective—To determine effects of all-trans and 9-cis retinoic acid (RA) on tumor growth and metastatic ability of canine osteosarcoma cells transplanted into athymic (nude) mice.

Animals—Forty-five 5-week-old female BALB/c nude mice.

Procedure—1 × 107 POS osteosarcoma cells were transplanted subcutaneously into the intrascapular region of mice. All-trans RA (3 or 30 µg/kg of body weight in 0.1 ml of sesame oil), 9-cis RA (3 or 30 mg/kg in 0.1 ml of sesame oil), or sesame oil (0.1 ml; control treatment) were administered intragastrically 5 d/wk for 4 weeks beginning 3 days after transplantation (n = 4 mice/group) or after formation of a palpable tumor (5 mice/group). Tumor weight was estimated weekly by measuring tumor length and width, and retinoid toxic effects were evaluated daily. Two weeks after the final treatment, mice were euthanatized, and number of mice with pulmonary metastases was determined.

Results—Adverse treatment effects were not detected. Tumor weight was less in mice treated with either dose of 9-cis RA than in control mice, although this difference was not significant. Treatment with 30 mg of 9-cis RA/kg initiated after tumor formation significantly reduced the incidence of pulmonary metastasis, compared with the control group.

Conclusions and Clinical Relevance—9-cis RA decreased the incidence of pulmonary metastasis in nude mice transplanted with canine osteosarcoma cells and may be a potential adjunct therapy for treatment of osteosarcoma in dogs. (Am J Vet Res 2000; 61:1241–1244)

Abstract

Objective—To determine effects of all-trans and 9-cis retinoic acid (RA) on tumor growth and metastatic ability of canine osteosarcoma cells transplanted into athymic (nude) mice.

Animals—Forty-five 5-week-old female BALB/c nude mice.

Procedure—1 × 107 POS osteosarcoma cells were transplanted subcutaneously into the intrascapular region of mice. All-trans RA (3 or 30 µg/kg of body weight in 0.1 ml of sesame oil), 9-cis RA (3 or 30 mg/kg in 0.1 ml of sesame oil), or sesame oil (0.1 ml; control treatment) were administered intragastrically 5 d/wk for 4 weeks beginning 3 days after transplantation (n = 4 mice/group) or after formation of a palpable tumor (5 mice/group). Tumor weight was estimated weekly by measuring tumor length and width, and retinoid toxic effects were evaluated daily. Two weeks after the final treatment, mice were euthanatized, and number of mice with pulmonary metastases was determined.

Results—Adverse treatment effects were not detected. Tumor weight was less in mice treated with either dose of 9-cis RA than in control mice, although this difference was not significant. Treatment with 30 mg of 9-cis RA/kg initiated after tumor formation significantly reduced the incidence of pulmonary metastasis, compared with the control group.

Conclusions and Clinical Relevance—9-cis RA decreased the incidence of pulmonary metastasis in nude mice transplanted with canine osteosarcoma cells and may be a potential adjunct therapy for treatment of osteosarcoma in dogs. (Am J Vet Res 2000; 61:1241–1244)